Journal of Natural Remedis

2-15

Authors: Fayyadhah Mohd. Azmi, S. Nagarajan MP Sockalingam, Mazlina Mohd Said, Ahmad Shuhud Irfani Zakaria

Studies on plant and food phytochemistry and its potential benefits to human health are becoming the focus of the research community. Researchers are turning to alternatives drugs in treating human diseases using natural products from plants and foods. Polyphenols are one of the largest groups in the plant family and consist of many subgroups. One of them is catechin, which is generally acknowledged to be part of a compound in tea. Over the years, investigations have shown that catechin has anti-oxidant, anti-inflammatory and antibacterial properties. In dentistry, documented evidence have shown the use of catechin in treatment of dental caries, periodontal disease, pulp pathology, and oral cancer. Other crucial areas of research include advancements in dental material incorporated with catechin. This review article explores the current studies on the potential use of catechin in dentistry.

29-41

Authors: R. A. Shastry, P. V. Habbu, D. M. Smita, Sudhir R. Iliger, V. H. Kulkarni

In the present investigation, we have carried out the isolation of fungal endophytes from Centella asiatica Linn leaves followed by fermentation and extraction of fungal endophytes with non-polar solvents such as chloroform, ethyl acetate and n-butanol. Preliminary phytochemical investigation of endophytic crude fractions of leaves were also determined to detect the presence of primary and secondary metabolites followed by invitro free radical scavenging activity by reducing power, DPPH and hydroxyl radical assay. The chloroform fungal endophytic fractions were subjected to column chromatography by gradient elution technique for isolation of possible secondary metabolite. Reducing power of endophytic extracts of C. asiatica Leaf (CAL-1) (50-450 μg/ml) increased with increase in concentration. Reaction with DPPH radicals of CAL-1 showed good scavenging activity. The IC₅₀ values for Ascorbic acid, chloroform extract, ethyl acetate extract and n-butanol extract were found to be 30.33 μg/ml, 66.58 μg/ml, 79.33 μg/ml and 96.39 μg/ml respectively. In hydroxyl radical assay, The IC₅₀ values for mannitol, chloroform extract, ethyl acetate extract and n-butanol extract were found to be 121.06 μg/ml, 141.21 μg/ml, 181.80 μg/ml and 189.90 μg/ml respectively. The endophytic crude fractions of ethyl acetate exhibited potent antioxidant activity as compared to other fractions. Hence, ethyl acetate fungal endophytic fractions of Centella asiatica Linn leaves can be employed as a potential antioxidant in the prevention of oxidative stress caused by the free radicals.

53-60

Authors: Balakrishnan Purushothaman, Nagarasan Suganthi, Kumaran Shanmugam

Ocimum basilicum L. (Lamiaceae) commonly known as basil possesses various medicinal activities to treat headache, diarrhoea, kidney malfunctions, diabeties, cancer and several pharmacological activities. These are mainly due to the various chemically diversified constituents present in it. In order to evaluate the chemical composition of basil leaves, the dried leaves were extracted with petroleum ether, chloroform, ethyl acetate, methanol and water by Soxhlet’s extraction. The extracts were screened for the qualitative determination by preliminary phytochemical screening. Quantitative determination was also performed for the total alkaloids, flavonoids, phenols, saponins and tannins. Amongst the extracts, methanol (MeOH) was identified to contain various classes of secondary metabolites, so GCMS analysis was performed on the MeOH extract. The results revealed that chemical constituents claimed for various biological activities were present.

68-69

Authors: M. K. Mohan Maruga Raja, Agilandeswari Devarajan, M. K. Kathiravan

No Abstract.