Biotechnologia Acta

5-18

Authors: N. I. ARALOVA, O. M. KLYUCHKO, V. I. MASHKIN, I. V. MASHKINA, T. A. SEMCHIK

The aim of the study was to develop a mathematical model to research hypoxic states in case of simulation of an organism infectious lesions. The model is based on the methods of mathematical modeling and the theory of optimal control of moving objects. The processes of organism damage are simulated with the mathematical model of immune response developed by G.I. Marchuk and the members of his scientific school, adapted to current conditions. This model is based on Burnet’s clone selection theory of the determining role of antigen. Simulation results using the model are presented. The dependencies of infectious courses on the volumetric velocity of systemic blood flow is analyzed on the complex mathematical model of immune response, respiratory and blood circulation systems. The immune system is shown to be rather sensitive to the changes in blood flow via capillaries. Thus, the organ blood flows can be used as parameters for the model by which the respiratory, immune response, and blood circulation systems interact and interplay.

32-41

Authors: V. B. KOVTUN, O. G. SIDORENKO, M. D. KHOMIAKOVA, I. A. LEVANDOVSKIY

The aim of the work was to analyze and summarize information on the functional activity of known retinoids, as well as to determine the directions of their modification and prospects for the use of their synthetic analogues in dermatology. The literature on retinoids metabolism stages in the human body, the mechanisms of their action and the properties that determine their use in various medical fields was considered and analyzed. Information on dermatological diseases treatment with natural retinoids and their synthetic analogues was summarized. The modern classification of retinoids based on the peculiarities of their chemical structure and functional activity is given, the relationship between these characteristics and the direction of use of such drugs in dermatology and cosmetology is shown. Prospects for the practical application of synthetic retinoids represented mainly by polyaromatic compounds are due to their higher stability and efficiency, as well as reduced irritant effect due to selectivity for nuclear receptors.

62-72

Authors: E. I. Yusova, T. V. Grinenko, T. O. Goncharenko, L. G. Kapustyanenko, A. A. Sobchenko, A. A. Tykhomyrov

Tissue plasminogen activator (tPA) is one of the key protein of plasminogen/plasmin system that converts plasminogen in the active proteinase plasmin. Platelets are able to bind both tPA and plasminogen on their surface, thus providing stimulatory effects on activation of zymogen. The present study was aimed to produce polyclonal antibodies against tPA and characterize their immunochemical capacities for further application in flow cytometry assay to study interaction between tPA and platelets. The experimental methods involved immunization of rabbit with tPA, collection of immune serum, synthesis of tPA-containing immunoaffine sorbent, ELISA, and flow cytometry. Polyclonal monospecific antibodies against tPA with high affinity to the antigen (Кd = 4.05・10–9 М) were obtained. Flow cytometry assay based on the use of the produced antibodies showed the presence of binding sites for tPA on the plasma membrane of inactive platelets. Moreover, agonist-stimulated platelets were revealed to expose more binding sites than their resting counterparts. Certain subpopulations of platelets that differ in the ability to bind tPA on their surface were also identified. Obtained data are of significant importance for further investigation of mechanisms underlying the role of platelets to regulate fibrinolytic rates.

87-100

Authors: T. V. Meleshko, O. V. Pallah, 2, R. O. Rukavchuk, L. S. Yusko, 2, N. V. Boyko

The aim of our study was to investigate the gut microbiota in patients with noncommunicable diseases associated with chronic inflammation, namely obesity, type 2 diabetes, atherosclerosis, and cardiovascular disease as well as to find out potential ability of edible plants’ fruits extracts to inhibit the growth of selected conditionally pathogenic microorganisms. Limited clinical trial was performed and gut microbiota analysis was done using routine methods and by qPCR. The antibacterial properties of edible plants’ fruits in relation to the selected potentially pathogenic microorganisms were studied. The composition of the intestinal microbiota of obese patients was characterized by an increase in the number of Enterococcus spp. and Lactobacillus spp. along with a decrease in the amount of Escherichia coli. Decreases in E. coli and lactobacilli were observed in patients with type 2 diabetes. In atherosclerosis, an increase in streptococci, enterococci, and enterobacteria was observed, whereas in patients with cardiovascular disease there was an additional increase in staphylococci and candida along with a decrease in E. coli. Decreases in Bifidobacterium spp., Bacteroides spp., Roseburia intestinalis and Akkermansia muciniphila were observed in patients of all groups. The growth of Klebsiella spp. was inhibited by red currant (Ribes rubrum) and plum (Prunus domestica) extracts; Enterobacter spp. – cherry (Prunus avium) extract; Proteus spp. – extracts of blueberry (Vaccinium myrtillus) and dogwood (Cornus mas); Staphylococcus spp. – the extracts of black currant (Ribes nigrum), cherry (Prunus avium), plum (Prunus domestica), jostaberry (Ribes nigrum × Ribes divaricatum × Ribes uva-crispa), cherry plum (Prunus cerasifera) and dogwood (Cornus mas) The obtained data can be used for early diagnosis of noncommunicable diseases and for their prevention with the help of personalized nutrition.