Biotechnologia Acta

5-27

Authors: T. P. PIROG, A. A. VORONENKO, M. O. IVAKHNIUK

Data on exopolysaccharides synthesis by psychrophilic fungi and bacteriae, halo- and thermophilic archaea and bacteriae, including those isolated from deep-sea hydrothermal vents — sources — were provided. Physiologic significance, physico-chemical properties and possible practical applications of exopolysaccharides from unusual sources were analyzed. Most of them have immunomodulating, antiviral, anticoagulant, antitumor, antioxidant activities promising for medical and pharmaceutical applications. Meanwhile, based on the literature date, the conclusion follows about the urgent necessity to develop efficient technologies for synthesis of these exopolysaccharides by nontraditional producers, which currently lags far behind common techniques.

50-56

Authors: O. V. Shtapenko, I. I. Gevkan, Yu. I. Slyvchyk, Ye. O. Dzen, V. Ya. Syrvatka, N. M. Matvienko

The purpose of this study was to investigate the effect of supplementation with organic zinc, manganese and chromium in the form of liposomal complex on the fertilizing ability and the level of antioxidant responses of female rabbits. Feeding of female rabbits with supplementation of organic forms of trace elements prior to insemination resulted in increase the numbers of corpora lutea, implantation and living fetuses compared to the control group. Moreover, there were the 4.4% and 1.7% decrease in pre- and post-implantation losses in animals receiving the organic microelements prior to insemination, respectively. The level of thiobarbituric-acid-reacting substances in ovary of experimental group was significantly higher (P ≤ 0.05) compare to the control group, while the level of lipid hydroperoxides in experimental group was decreased. In the uterus of rabbits after addition organic compound of trace elements significantly decreasing the thiobarbituric-acid-reacting substances level was by compare to the control animals (P ≤ 0.001). The level of the superoxide dismutase activity in uterus and ovary of female rabbits in the experimental group were significantly higher than in the control group (P ≤ 0,01). Our studies indicated that supplementation organic microelements in liposomal form to the basal diet for 2 weeks before insemination had a beneficial effect on the metabolism intensity and maintaining antioxidant-prooxidant balance in reproductive organs that improve fertilization and mbryo implantation.

68-72

Authors: O. B. Zlenko, A. P. Gerilovych

The aim of the work was to construct and test the recombinant positive control for F. tularensis detection by a real-time polymerase chain reaction (qPCR). The molecular TA-cloning of pTZ57_F/R plasmid ligated with tul4 gene PCR product into DH5α E. coli. The minimal detection level in qPCR was one copy number per reaction. The obtained positive control was highly sensitive, specific and safe qPCR in the laboratory tularemia diagnostics.

84-90

Authors: Y. Hurmach, М. Rudyk, V. Svyatetskaya, L. Skivka

The aim of the work was to investigate the effect of the intracranial catheter placement on the metabolic profile of rat microglia. Microglial cells were isolated by the centrifugation in Percoll gradient. Oxidative metabolism and phagocytic activity were investigated by flow cytometry. Arginase activity was examined by colorimetric method. Nitrite level was assayed in Griess reaction. It was found that intracranial catheter placement caused down-regulation of nitrite synthesis by 3 times, augmentation of the reactive oxygen species generation by 1.5 times, and slightly decreased microglia phagocytic activity. Thus, intracranial catheter placement causes long term anti-inflammatory shift of microglia metabolism in rats.