Biotechnologia Acta

26-37

Authors: D. O. Tsymbal, D. O. Minchenko, O. O. Riabovol, O. O. Ratushna, O. H. Minchenko

We have studied the expression of genes encoding proliferation related factors and enzymes such as IL13RA2, KRT18, CD24, ING1, ING2, MYL9, BET1, TRAPPC3, ENDOG, POLG, TSFM, and MTIF2 in U87 glioma cells upon glucose and glutamine deprivation in relation to inhibition of inositol requiring enzyme 1, a central mediator of endoplasmic reticulum stress. It was shown that glutamine deprivation leads to up-regulation of the expression of BET1, MYL9, and MTIF2 genes and down-regulation of CD24, ING2, ENDOG, POLG, and TSFM genes in control (with native IRE1) glioma cells. At the same time, glucose deprivation enhances the expression of MYL9 gene only and decreases – ING1, ING2, and MTIF2 genes in control glioma cells. Thus, effect of glucose and glutamine deprivation on gene expressions in glioma cells is gene-specific. Inhibition of inositol requiring enzyme 1 by dnIRE1 significantly modifies the effect of both glutamine and glucose deprivation on the expression of most studied genes with different direction and magnitude, especially for ING2, CD24, and MTIF2 genes. Present study demonstrates that IRE1 knockdown modifies glucose and glutamine deprivation effects on the expression of proliferation related genes and possibly contributes to slower tumor growth of these glioma cells after inhibition of IRE1 signaling enzyme.

48-54

Authors: D. R. Аbdulina, L. М. Purish, G. А. Iutynska, М. М. Nikitin, A. G. Golikov

The possibility of the designing test-systems for specific detection of corrosive-relevant sulfate-reducing bacteria using real-time PCR assay were investigated. This method of the bacteria identification is based on the detection of the functional genes, encoding key enzymes of dissimilatory sulfate-reduction pathway, i.e. dissimilatory sulfitreductase α subunit dsrA. It was established among the six test-systems specificity reveal only three designed on the base of Desulfotomaculum, Desulfovibrio, Desulfobulbus genera sequences. The most corrosive-relevant strain Desulfovibrio sp. UCM B-11503 dsrA gene detected more effectively (threshold cycle was 20,0), than less corrosive-relevant strains Desulfovibrio sp. UCM B-11504 (threshold cycle was 28,1) and for Desulfotomaculum sp. UCM B-11505 and Desulfomicrobium sp. UCМ B-11506 were 24,9 and 23,1 cycles, respectively. Test-systems allowed identifying corrosive-relevant sulfate-reducing bacteria faster and more effective. This approach will serve as a base for monitoring of these bacteria for estimating corrosion sites on the high-level dangerous man-caused objects.

64-70

Authors: L. D. Varbanets1, А. N. Kyrychenko, I. I. Seifullina, N. V. Shmatkova, О. S. Brovarskaya, L. V. Yaroshenko, L. D. Popov

The purpose of the research was to investigate changes of antiviral activity of Pseudomonas chlororaphis subsp. аureofaciens lipopolysaccharides (LPS) as a result of their modifications by coordination compounds of Sn(IV) and Ge(IV) synthesised on the basis of aromatic, pyridinecarboxylic acids hydrazides and appropriate hydrazones of aromatic aldehydes. A wide range of Ge(IV) and Sn(IV) coordinative compounds was chosen for P. chlororaphis subsp. аureofaciens UCM-306 LPS modification. «Tobacco mosaic virus – hypersensitive plant» model study of LPS and its modified preparations (1-35) antiviral activity showed that a lot of the tested preparations exhibit high antiviral activity due to their composition and structural peculiarities. Such preparations are of interest as perspective agents in struggle against plant virus diseases. Antiviral action of preparations (% of inhibition, I,%) which neutralizes virus infectivity, depends on: complexing metal [complexes of Sn(IV) are more active (I,% 65-79) as compared to Ge(IV) complexes (I,% 26-62)]; amount of functional groups in ligand molecules [hydrazone complexes of Sn(IV) are more active (I,% 69-79) then hydrazide ones (I,% 48-63)]; and also on coordination form of ligand coupling with different substituents [complexes with salicyloyl hydrazones of 4-mеtoxy- (I,% 71), 4-hydroxybenzaldehydes (I,% 77) and pyrogallol (I,% 72) with ketone O(C=O)–N(CH=N) form of a ligand are more active than with enol O(C-O)–N(CH=N) form of isonicotinoyl hydrazones of the same aldehydes (I,% 32-63)]. Introduction of two substituents (OH- and Br-) into the hydrazide fragment of hydrazone molecule significantly increases the activity of Sn(IV) complexes with enol form of the ligand (I,% 79).

79-86

Authors: T. Z. Moskalets, S. P. Vasylkivskyi, B. V. Morgun, V. І. Moskalets, V. V. Moscalets, V. K. Rybalchenko

The aim of the research was to conduct basic screening of new lines and cultivars of winter hexaploid triticale by the technological and molecular genetics indicators. Molecular and genetic research conducted by polymerase chain reaction allelic variants of gene loci Wx-A1, Wx-B1, and quality parameters of grain, flour and bread – on technological markers. The new cultivars and lines of winter hexaploid triticale of Nosivka Breeding and Research Station of Remeslo Myronivka Institute of Wheat by technological indicators of grain, flour and bread quality were studied. According to representative criteria’s the most promising genotypes, which are the main products in terms Forest-Steppe ecotypes’ and a high-quality raw materials for bakeries and bioethanol were identified. Molecular and genetic identifications of allelic variants of genes loci Wx-A1, Wx-B1 triticale, which in the early stages of ontogenesis to predict targeted uses genotypes were conducted. The first among a series of triticale cultivars and lines Forest-Steppe ecotypes and biotypes with nonfunctional b gene allele WxA1, which defines a high content of amylopectin of starch, an important release for more ethanol was identified. It was found that technological characteristics of grain, flour and bread of new cultivars and lines of winter triticale meet the modern requirements production dietetic food and bioenergy products is important and relevant in the context of food security of Ukraine.

97-102

Authors: L. S. Dorosh, T. B. Peretyatko, S. P. Hudz

The aim of research was to study nitrate reductase activity of sulphate-reducing bacteria Desulfomicrobium sp. CrR3 at the different conditions of cultivation. To determine nitrate reductase activity, sulphate-reducing bacteria Desulfomicrobium sp. CrR3 cultivated in a modified Postgate C medium. Nitrate reductase activity was determined at different conditions: to 15–45 °C, pH 5–9. The highest nitrate reductase activity for Desulfomicrobium sp. CrR3 was at to 25 and 35 °C and pH 7–8. Lowering or raising the temperature and pH caused the inhibition of the nitrate reductase. The highest nitrate reductase activity was found in the soluble fraction of cells (12 µmol nitrite•min-1•mg protein-1). Activity was lower in the cells debris (5 µmol nitrite•min-1•mg protein-1), while it was absent in the culture fluid. It was established that nitrate reductase of bacteria Desulfomicrobium sp. CrR3 is constitutive enzyme. The value of Km for nitrate for studied enzyme in bacteria Desulfomicrobium sp.CrR3 is 1.2 µmol, Vmax – 15.7 µmol nitrite•min-1•mg protein-1), indicating that the high affinity of the enzyme to the substrate. Thus, the cultivation conditions significantly affect the nitrate reductase activity of bacteria Desulfomicrobium sp. CrR3.