VITALITY AND MORPHOLOGY OF TUMOR CELLS TREATED WITH 4-TIAZOLIDINONE DERIVATIVES IMMOBILIZED ON NANOSCALE POLYMER CARRIER
Authors: N. M. Boiko, O. Yu. Klyuchivska, L. I. Коbylinska, D. Ya. Havrylyuk, A. O. Ryabtseva, N. Ye. Mitina, R. B. Lesyk, O. S. Zаichenko, R. S. Stoika
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A nanoscale polymeric carrier was used for delivery of novel anticancer compounds – 4-tiazolidinone derivatives – to tumor cells of different lines. It was found that such way of delivery of the above mentioned compounds to target cells significantly (approximately 10 times) decreased acting cytotoxic dose of some of these compounds with preservation of similar level of their antineoplastic effect in vitro towards various mammalian tumor cells. The microscopic investigation of these cells demonstrated that under the action of some immobilized 4-tiazolidonone derivatives, there was an increase (up to 40%) of the part of apoptotic cells, as well as an appearance of 10% of cells with morphologically changed nucleus, and up to 35% of cells with an increased intensity of red fluorescence of acridine orange in the lysosomes, compared with such indicators observed under the action of free form of those compounds. Thus, the applied nanoscale carrier is a perspective polymer system for delivery of anticancer drugs to target cells.