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Modified Nanodiamonds as a New Carrier for Developing Reusable Enzymatic Test-Systems for Determination of Physiologically Important Substances
Authors: Nikita Ronzhin, Alexey Baron, Alexey Puzyr, Irina Baron, Andrey Burov, Vladimir Bondar
Number of views: 521
The study demonstrates the application of detonation nanodiamonds in designing testsystems
for determination of physiologically important blood substances. One (urease), two (glucose
oxidase and peroxidase) and three (cholesterol esterase, cholesterol oxidase and peroxide) enzymes
have been covalently immobilized onto the surface of modified nanodiamonds (MND) to develop testsystems
for biochemical detection of urea, glucose, and total cholesterol, respectively. In all cases, the
immobilized enzymes exhibit functional activity and catalyze the formation of a colored product in
the presence of the analyte. It has been found that the obtained MND-enzyme(s) complexes
effectively function in deionized water and different buffers providing a linear output of the final
product over a wide range of temperatures, pH and analyte concentrations. The model biosensors
were found to be suitable for repeated use and retain most of their activity after storage at 4 °C in
deionized water for two months. Practical applicability of the glucose and total cholesterol biosensors
is demonstrated in human serum samples.