Study the synergistic effect of virotherapy and chemotherapy on tumor cells in vitro
Authors: Nahi Y. Yaseen, Ahmed M. Al-Shammari
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Virotherapy, by using Newcastle disease virus, is a successful anti-tumor agent for in vitro studies. As with other modalities of treatment, oncolytic virotherapy, by itself, has not been effective in complete tumor eradication in both preclinical animal models and clinical studies. It appears that the best chance for complete tumor eradication lies with combining its mechanism of action with current treatment strategies of chemo- and radiation therapies and the emerging field of clinical gene therapy. The theory, based on combination chemotherapy, is that attacking tumor cells through different mechanisms of action will prevent tumor cells from having time to develop resistance to treatment. Oncolytic viruses can engender an increased sensitivity of tumor cells to chemotherapy and radiotherapy, so we investigate the possibility of synergistic effect between Newcastle disease virus and chemotherapy in vitro. In the current study we used cytotoxicity assay and combination index to determine the most synergistic chemotherapeutic agent with Newcastle disease virus Iraqi strain, we used panel of different chemotherapies, and tested this combination for apoptosis induction. This combination tested for safety on normal cells. The results showed that Cyclophosphamide work in synergism with NDV as shown in cytotoxicity and apoptosis assays also it was safer when tested on normal cells. This study encourage to move for in vivo study to determine if Cyclophosphamide and NDV are powerful if used to treat transplanted tumors in mice.