Current Research in Pharmaceutical Sciences

55-62

Authors: N. Vijaykumar

Immunoprotective activity of Septilin evaluated against Cyclosporine A induced immunosuppression in wistar albino rats. An equivalent of 100mg of Septilin tablet extract administered as an oral dose and its Immunoprotective activity assessed by determining of host resistance against E.coli, determining hematological parameters (Total Leukocyte Count), determining in vivo phagocytic test by carbon clearance, determining humoral response (by measuring antibody titre) and cell mediated immunity (by measuring foot pad thickness). The present study suggests that Septilin significantly protected the animals by increasing the immunoprotective activity in immune compromised conditions. In Septilin treated, immunosuppressed rats, there was 33.3% mortality against 100% mortality in immunosuppressed rats after 48hrs of infection. 16.6% mortality was observed in Septilin treated rats against 66.6% in immunosuppressed rats after 24hrs of infection. Furthermore, Septilin treatment raised the TLC significantly (P<0.01) when compared with control immunosuppressed rats. It was also found that Septilin treatment potentiated the phagocytic activity in immunosuppressed animals. The antibody titre determination study suggested that Septilin enhances both primary and secondary responses in rats immunized with BSA. In normal rats, Septilin increased the primary antibody titre values by 2.4 times and the secondary antibody titre values by 1.56 times. In immune suppressed rats, Septilin increased the primary antibody titre values by 2.5 times and secondary antibody titre values by 1.81 times. Septilin also showed the protective activity against impaired DTH conditions.

68-73

Authors: Neelkant Prasad, Roshan Issarani, Badri Prakash Nagori and Amar Deep Singh

This article describes the development of UV spectrophotometric method for detection of glibenclamide during its dissolution in alkaline borate buffer pH 9.5 because no official UV spectrophotometric method is described for this drug and no such method has been developed for estimating the drug in this medium. A stock solution of 20 g/ml was prepared. Various aliquots of the stock solution were transferred to a series of 10 ml capacity volumetric flasks so as to prepare various dilutions between the ranges of 2-12 g/ml. To each dilution 0.1 ml of concentrated hydrochloric acid was added and a calibration curve was formed at 229.5 nm between absorbance on Y- axis and concentration on X- axis. The linearity range was found to be between 2.0 to 12.0 g/ml. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.23 g/ml and 0.71 g/ml respectively. The repeatability, interday and intraday precision (measured in terms of percent relative standard deviation, %RSD) was always less than 0.94%. The accuracy of the developed method were measured by recovery studies and was found to be between 100.39%±1.03% and 102.94%±1.46%. Thus, the developed method is specific, accurate and precise as validated according to recommendations by ICH guidelines.