01-12
Recycling Paper Waste for Sustainable Sugar Production / Kirkuk-Iraq
Authors: Asal Aziz Tawfeeq and Mohammad Aljaradin
Number of views: 377
In the work, fifty soil samples were cultivated on selective Gauze agar medium plates
containing carboymethyl cellulose as the sole carbon source. Thirty-two samples showed
similarity to the morphological characteristics of Streptomyces bacterium which were tested for
cellulolytic activity using the 3,5-Dinitrosalicylic acid reagent. Four efficient Streptomyces
isolates were selected for their high cellulolytic activity which was recorded after 12 hours of
incubation in the early logarithmic phase of growth. The four isolates attained their optimum
cellulose activity after 96 hours of incubation with an enzyme activity of (0.55 + 0.1, 0.53+ 0.12
and 0.49 + 0.22 U/mL) by the Streptomyces isolates AA-17-1, AA-17-19 and AA-17-22
respectively with recording a specific enzymatic activity of (0.68 + 0.23 U/ml) of the
Streptomyces isolate AA-17-32. The result showed, a supreme activity of the Streptomyces
purpureus AA-17-32 to reduce (20 g) of paper waste to about (0.78 U/ml). After applying
further morphological and biochemical analysis it’s recognized that, the Streptomyces isolate
AA-17-32 belonged to the species Streptomyces purpureus. It concludes that this method for the
production of cellulose can be used for the sustainable development of sugar production from the
degradable of waste materials.