International Journal of Applied Sciences and Biotechnology

394-399

Authors: Bijay Kumar Shrestha, Jenish Shakya

Candida albicans is a member of the healthy human microflora, colonizing several niches in the body and can cause opportunistic infection under host debilitated and immunocompromised condition. The present study aimed to investigate the in-vitro hemolytic activity of C. albicans isolated from oral cavity and screen biofilm through three different methods. During the study, 200 oral rinse samples from general human population were analyzed in microbiology laboratory of Central Campus of Technology, Tribhuvan University, Hattisar, Dharan. Nepal. Candida albicans were isolated and identified by conventional microbiological procedures. The hemolytic activity was evaluated through two different Sabouraud dextrose broth media (SDB) containing 7% defibrinated human blood, one supplemented with 3% glucose (SDBwG) and the other without glucose (SDBwoG). The biofilm formation was screened through congo red agar, tube method and tissue culture plate method. In this present study, 42 (21%) isolates of Candida albicans were isolated from 200 oral rinse samples. Isolated Candida albicans exhibited mean hemolysis activity of 28.66% on human blood SDB without glucose and 43.55% on human blood SDB with 3% glucose. Tissue culture plate method was considered sensitive, specific and accurate method for quantitative screening of biofilm in comparison to tube method and congo red agar method. This research concluded that Candida albicans exhibited greater hemolytic activity in human blood with glucose (SDBwG) than in human blood without glucose (SDBwoG). This finding explains that an increased blood glucose concentration may contribute to increased hemolysis activity of Candida albicans that could play pathogenic role for inducing infection like oral candidiasis in debilitated host like diabetic patients. Tissue culture plate method can accurately screen biofilms than tube and congo red agar method.

410-416

Authors: Srinivasa Murthy Kotilingam

Identification of termites is a challenging task due to variable morphological differences among species and their eusocial behaviour with different caste system. A method for easy and accurate species-level identification at any life stage is required. In this study, a 658-base pair region of the mitochondrial cytochrome oxidase I (COI) gene was used to explore its utility in the identification of important subterranean termites. Termite specimens collected from 25 locations from various states, were characterized using specific primers for their identification. Sequence analysis and divergence among the species was assessed. The composition of the mitochondrial sequence of the COI gene in the present study was expectedly AT biased. GenBank accession numbers were obtained for the species. Sequence analysis and divergence among the species was assessed, which revealed significantly high percentage of A+T base composition. Molecular sequence information from NCBI revealed relatedness in all the collected termites, accurately as revealed by their morphological characters. The studies indicate the relevance of DNA sequencing to match different forms of termites and address ambiguities in morphological identification. The information on species diversity would help plan strategies for management of termites.

422-426

Authors: Pushpa Karna Mallick

In present investigation karyotype of mitotic chromosomes were determined, two species of the genus Spilanthes, namely Spilanthes acmella (L.) Murray and Spilanthes calva DC. from plants transplanted in earthen pots collected from different localities from Central Nepal in my home garden. The somatic chromosome number determined in present study were 2n=36 for both species, Spilanthes acmella (L.) Murray and Spilanthes calva DC. The chromosomes total length ranged from 0.6 to 1.6 µm in Spilanthes acmella (L.) Murray and 0.4 to 2.1µm in Spilanthes calva DC. The relative length ranged from 2.3 to 12.1 µm in the species Spilanthes calva and 2.0 to 8.5 µm in the species Spilanthes acmella. The Karyotype formula were M16+m2+sm16+st2 in Spilanthes calva DC. and M22 +sm14 in Spilanthes acmella (L.) Murray cytologically obtained by using 2% aceto-orcein. The karyotype of the two species shows considerable variation exists in morphology of chromosome and structure. The variation in karyomorphology shows evolved nature of this species which play a great role in evolution.

432-436

Authors: Prashant Bhatt, Pratibha Bist, Laxmi Narayan Ojha

Kailali is the second highest district regarding wheat production and area coverage. A study was carried out in the Kailali district for preference of farmers on different wheat varieties that were being cultivated in the district. The objective of the study was to assess farmers’ preference and perception on the different improved wheat varieties in the area. Five commonly grown wheat varieties and seventeen important wheat varietal traits were selected through focus group discussion, key informant interview and preliminary survey. Sixty households were selected for face-to-face personal interview, twelve for each selected variety, at random from Ghodaghodi Municipality, Gauriganga Municipality and Kailari Rural Municipality of Kailali. The weightage of the seventeen selected traits were determined using an online survey conducted among individuals from different designation with the agricultural background who were well known about wheat cultivation practices in Kailali district. Yield, flour quality and tillering were found to be the trait of higher weightage and stem thickness was the trait of lowest weightage. The preferences of the farmers were measured using wheat preference index (WPI). The varieties Vijay, NL-971, HD-2967, Gautam and Aditya were ranked as I, II, III, IV and V respectively. The incorporation of farmers' preferred traits in future wheat varieties would increase the likelihood of adoption of the varieties. Similarly, proper participatory field trial of new improved wheat varieties would also help in the selection of the suitable varieties in the area concerning the farmers’ criteria of selection.

448-453

Authors: Prakash Acharya, Punya Prasad Regmi, Devendra Gauchan, Dilli Bahadur KC, Gopal Bahadur KC

A study was conducted in Jhapa, Sunsari and Bardiya district of Nepal to assess the benefit cost (BCA) analysis of small farm machineries (transplanter, reaper and power tiller) used for rice cultivation. Out of total respondents of 274 under mechanized farm category selected using Raosoft Software of sample size determination, 74% reaper owner (20), 67% power tiller owner (20) and 100% transplanter owner (09) were selected for analyzing benefit cost analysis using simple random sampling. BCA analysis showed that the NPV, B/C ratio, IRR and Payback Period of investing in transplanter were NRs 452743.62, 1.61, 24% and 2.75 years at 12% discount rate respectively. Similarly, NPV, B/C ratio, IRR and Payback period for reaper and power tiller were NRs 422541.93, 2.89, 123% and 1.14 years and NRs 619,719.34, 2.32, 65% and 1.46 years at 12% of discount rate respectively. The investment on reaper and power tiller would be profitable for their higher Benefit Cost ratio and IRR, and lower payback period. The results of sensitivity analysis showed that investments in reaper and power tiller would be profitable even if decrease in benefit or increase in cost or decrease in benefit and increase in cost by 20% is considered. However, in case of transplanter, the IRR would be less than the discount rate when benefit decreases by 20% and cost increases by 20%. Due to high investment at the initial stage, the payback period was longer and IRR was also less than 30% per annum in transplanter which indicated that investment would not be made for transplanter unless price of transplanter is lowered through regulation of price and provision of subsidy. It is suggested to motivate farmers for adoption of small farm machineries in rice cultivation through provision of differentiated rates of subsidy and technical capacity build up.