390-396
Molecular Identification of Synanthropic Flies in Malaysia
Authors: 1,2Khoso, F. N, 3Wong, S. K, 4,5Chia, S. L and 1Lau, W. H
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This study was conducted to determine the presence of filth feeding and breeding flies
at markets in Serdang, Malaysia. A total of 1,158 flies were collected during the study
period and 14 species were successfully sequenced and identified. The identified
species were belonged to three main families, Calliphoridae (Chrysomya rufifacies,
Chrysomya megacephala, Hemipyrellia ligurries and Lucilia cuprina), Muscidae
(Musca domestica, Musca ventrosa, Ophyra chalcogaster, Ophyra spinigera and
Synthesiomyia nudiseta) and Sarcophagadae (Sarcophaga dux, Sarcophaga peregrina,
Sarcophaga ruficornis, Sarcophaga taenionota and Sarcophaga kempi). Intraspecific
variation ranged from 0.00% to 1.05%, and interspecific variation occurred between
5.82% and 13.82%. The phylogenetic tree revealed that all species formed distinct
monophyletic clades. In addition, the COI gene sequence of Musca ventrosa and
Ophyra chalcogaster was reported first time ever in Malaysia.