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Improving the Performance of Nano–Endofullerenes in Polyaniline Nanostructure–Based Biosensors by Covering Californium Colloidal Nanoparticles with Multi–Walled Carbon Nanotubes
Authors: Alireza Heidari
Number of views: 492
In the current paper, Glucose (Dextrose), Fructose and Galactose–oxidase enzymes are
used as stabilization medium due to its more efficiency, ability for more accurate controlling the
enzyme reaction, protecting against wasting of enzyme as well as simple and easy use and exchange
of enzyme medium after performing some levels of surface modification and developing Nano
Endohedral Fullerenes (Endofullerenes) on Californium plate. For better connecting and stabilizing
the enzyme on the medium, the prepared medium is washed by high concentration Sulfuric Acid and
Nitric Acid and a large volume of deionized water and for protecting enzyme from devastating effect
of Californium and prohibiting them to become inactive, surface is covered with Cystamine before
stabilization. Regarding the large size of Glucose (Dextrose), Fructose and Galactose–oxidase
enzymes compared to surface of medium, a connective material with amid at one end and Pyrine at
the other end is used as transfer agent and for stabilizing this connection, the prepared medium is
placed into dimethylformamide (DMF) solution for a couple of hours. Activity of stabilized enzyme
at 460 (nm) wavelength recorded by spectroscope was depicted against time to evaluate its stability
in various times. The prepared medium, which has a large amount of Glucose (Dextrose), Fructose
and Galactose–oxidase enzymes, can be used as electrode in sensors. Furthermore, qualitative and
quantitative measurement of food components is of great importance due to high cost of traditional
methods, in addition to tendency for more accurate and sensitive detecting of these components.
Glucose (Dextrose), Fructose, Galactose and Cholesterol and Cholesterol are such compounds that
they frequently measure. Various methods are used to detect these food elements. However, the
necessity for accurate measurement of these two compounds with high sensitivity, especially for food
health issue, leads to developing biological methods, especially biosensors. Among them, biosensors
based on conductive polymer nanostructures, especially Polyaniline (PANI), have been recently
interested due to their unique characteristics. The current paper aims to introduce and investigate
the previously performed studies about Polyaniline (PANI)–based biosensors for detecting Glucose
(Dextrose), Fructose, Galactose and Cholesterol and Cholesterol. In addition, Glucose (Dextrose),
Fructose and Galactose–oxidase electrochemical sensor is one of the best methods for detecting low
amount of Glucose (Dextrose), Fructose and Galactose and applying Californium colloidal
nanoparticles as a supplementary material in the structure of biosensor can be effective for improving
its efficiency and optimum performance.