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Prevalenceand Antibiotic Resistance of Pathogenic Bacteria Isolated from Diabetic Foot Infections
Authors: Alsadig Mohammed Abdalla, Abu sabeeb AlbasherAlsheik, Hossam Aldean Mustafa Dyab , Zahra Ahmed
Number of views: 816
The increasing incidence of antibiotic resistant pathogen as cause of diabetic foot infection makes selecting empiric antibiotic therapy more difficult. Those who treat these patients are well aware of the growing problem of Methicillin-resistant Staphylococcus aueus (MRSA). The aim of this work was to study the relative frequency of bacteria isolated from diabetic foot infection and assess their comparative susceptibility to the commonly used antimicrobial agents. A total number of 50 cases of diabetic foot infection patients attending at Khartoum state hospitals were investigated to isolate bacterial pathogens responsible for diabetic foot infection and to test their antimicrobial susceptibility patterns. Samples were collected by swabbing from all studied patients and examined by Gram stain, cultured on Blood agar, Mannitol salt agar, MacConkey agar. Biochemical and antimicrobial susceptibility tests were done. The species of bacteria isolated were: Staphylococcus aureus 32%, Pseudomonas aeruginosa 14%, Klebsiella pneumoniae 12%, Escherichia coli 10%, Proteus vulgaris 10%, Proteus mirabilis 8%, Staphylococcus epidermidis 8%, Citrobacter freundii 4%, Enterobacter species 2%. The antimicrobial susceptibility testing revealed that Amikacin was the most effective drug against both gram positive and gram negative bacteria followed by Gentamycin. 62.5% of the S. aureus isolates were resistant to Methicillin. All S. epidermidis isolates were resistant to Methicillin, Erythromycin, Ceftriaxone, Gentamicin, and Trimethoprim. All gram negative isolates were resistant to Ampicillin.