Annales Bogorienses

1-7

Authors: Ines Atmosukarto, Grant W. Booker

Signal transduction cascades are critical components of intra- and inter-cellular communication. Key component of such cascades includes tyrosine kinases. One such family of tyrosine kinases is the Tec family of tyrosine kinases . The family of tyrosine kinases is expressed mainly in cells of the hematopoietic lineage, and mutations in at least one of its one member of this family, Btk, has so far been asociated with the human immunodeficiency disorder X-Linked Agammaglobulinemia. Two major isoforms of the Tee transcript, referred to as TecIII and TecIV has been detected in various mouse embryonic and adult tissues, as well as in a number of different hematopoietic cell lines: Tec IV is the full length Tec protein with functional PH, TH, SH3 , SH2 and Kinase domains, while TecIII is generated by the splicing out of exon 8 sequences to yield a shorter peptide with a non-functional SH3 domain. Using GFP-TecIII fusion proteins, this shorter isoform ofTec was shown to have biological characteristics that differed from TECIV.

15-22

Authors: Andi Utama, Hiroyuki Shimizu

Recent outbreaks of circulating vaccine-derived polio virus (cVDPV) revealed the possibility of recombination between vaccine strains poliovirus (PV) and cluster C enterovirus . Based on genetic analysis, it is assumed that coxackie virus 11 (CAV-11 ), one of the cluster enterovirus, may naturally recombine with PV. To elucidate this hypothesis , the chimeric virus between PJ156, a type 1 cVDPV isolate isolated from an acute flaccid paralys is (AFP) case in the Philippines in 2001 , and CAV-11 (PJI56/CAV-11) was constructed by using long-PCR method . As the result , PJl56/CAV-11 was aviable in HEp-2 cell line. The PJl 56/CAV-11 exhibited mostly similar phenotype with parental PJ156 in term of plaque size, viral growth and neurovirulence. These results suggested that recombination between PV and CAV-11 might naturally occur during transmission of vaccine strains in the community. The effect of recombination on the viral phenotype is significantly depending on the counterpart virus

31-39

Authors: Tatik Khusniati, Yantyati Widyastuti

Pasteurized milks spoiled at refrigerated storage due to growth of psychrotrophic bacteria. Honey which contain antibacterial and aromatic compounds may be used as supplement to inhibit psychrotrophic bacteria activitiy . To know nutritional and flavor compounds of milks with and without honey, effect of honey on bacterial growth0protein degradation. amino acids and volatile compounds of stored milk were detected. Bacterial growth,protein degradation, amino acids and navor compounds were detected by total plate counts. formol titration,HPLC and GCMS, respectively. The results show that bacterial growth and protein degradations in honey milks were lower than that without honey. Bacterial growth (5.2x10^3 - 9,3x10^6 cfu/mL) and protein degradation (2,37-2.59%) in honey whole milks were lower than that (6.2x10^4 - 6.5x10^7 cfu/mL)(2.54-2.88%) in skim milks,respectively (P<0,05). At 10 days after use by date, changing between amino acids' contents in whole milks withand without honey were more significant than that or skim milk (P<0.05);. and volatile compounds' percentages in honey whole milks were higher than that without honey. while that in honey skim milks vice versa. Honey caused decreasing bacterial growth and protein degradation, changing aminoi acid contents and producing volatile compounds of stored milk, and honey whole milk were better than honey skim milks.