NOVA

7-16

Authors: Leonardo Yunda, David Pacheco and Jorge Millan

Objective: a Web-based Fuzzy Inference Tool for cardiovascular risk assessment has been developed. The tool makes use of inference rules from evidence-based medicine for membership classification. Methods: the system framework allows adding variables such as gender, age, weight, height, medication intake, and blood pressure, with various types of membership functions based on classification rules. Results: the tool allows health professional to enter patient clinical data and obtain a prediction of cardiovascular risk. The tool can also be later used to predict other types of risks including cognitive and physical disease conditions.

27-38

Authors: Luz Mabel Ávila Portillo, Derly Johanna Franco Ruiz, Jenniffer Priscila Avila García, Angela Giselle Riveros Arocha, Mauricio Sabogal

Objective. Comparing viability, growth in culture, phenotype and osteogenic lineage differentiation of Adipose-Derived Adult Stem Cells (ADAS) pre and post freezing. Methods. Samples were obtained from adipose tissue of healthy individuals who underwent liposuction, to which underwent enzymatic digestion with collagenase I. The stromal vascular fraction (SVF) obtained was divided equally into two fractions one of which was cryopreserved and stored at -196 °C and another cultivated to passage 1 (P1) before being cryopreserved. Both fractions were cryopreserved by chamber automated freezing using two solutions: (A) DMSO/Dextran40/HSA and (B) DMSO/HESSICO/HSA kept 3 months at -196°C and thawed in PBS/HSA/HESSICO. After its thawing were also subjected to cultivation, count, cell viability, phenotyping and osteogenic potential. Results. Found that frozen cells with cryoprotectant solution DMSO/Dextran40/HSA and a pre-freezing culture, viability percentages exhibited higher and reached 80% confluence in less time. Expanded samples showed no differences in the phenotype by flow cytometry and shown to osteogenic lineage differentiation.

47-54

Authors: Jhonatan Betancourt-Peña, Beatriz Elena Muñoz-Erazo, Hugo Hurtado-Gutiérrez

Objective. To describe a pulmonary rehabilitation program impact in patients with sequelae of pulmonary TB. Methods: A quasi-experimental study in eleven patients in a pulmonary rehabilitation program with continuous exercise, muscle strengthening and education during eight weeks; it was evaluated before and after the BMI, dyspnea, six-minute walk test, anxiety, depression, and quality of life related to health; paired t was used to analyze measures. Results: eleven patients completed the pulmonary rehabilitation program. The average age was 21.7 ± 45.4 years. Eight patients were male. By the end of the program, a significant increase was found in the walked distance in the 6MWT with an average difference of 110.2 ± 112.5 meters (p = 0.009). Health-related quality of life had a significant decrease in the total score in SGRQ, an average difference of 6.7 ± 8.3 (p = 0.02), no significant differences were found in the other domains of the questionnaire. There is a good exercise tolerance in patients with sequelae of TB in pulmonary rehabilitation. Physiotherapists must consider the difference in the distance covered in the six-minute walk test by the beginning and the end of the program as a reliable variable related to the increased of the functional ability.

93-99

Authors: Elías Alberto Bedoya Marrugo

Introduction: labor accidents is linked to a number of factors , including a lack of risk control at work, Occupational Risk Administrators do not make the payment on promotion and prevention and especially employers who violate legal requirements safety and health at work, further exacerbating the current situation. Objective: to establish the behavior of the accident of a company in the metalworking sector in the periphery of the city of Cartagena, during the 2014 period. Method: review of accident data from a engineering company during the years 2014 , next to a discrimination of the types of accident and characteristics of the charges affected class , type of linkage of those affected, types of injuries, mechanisms of injury and accidents disabling accident charge , age and place of occurrence. Result: a total of 36 accidents in which the prevalences were found among others: office assistant plant affected with 33 %, 22 % trainee, 75 % of those affected are in the company, while the remaining 25 % belong to contractors, being the month of October and April with the roughest 22.2 % respectively limb wounds with 66 % are the most common type of injury, followed by crushing 27%. The accidents reported in this research significantly affect workers in positions of apprentice and assistant plant, as it mainly affected the upper and lower extremities (hands and feet).