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COMPARATIVE MODELING OF CDP-DIACYLGLYCEROL-SERINE O-PHOSPHATIDYLTRANSFERASE IN CLOSTRIDIUM BOTULINUM: A POTENT TARGET OF BOTULISM
Authors: Koteswara Reddy G., Mohan Kalyan Reddy K., Nagamalleswara Rao K., Gyana R.Satpathy
Number of views: 363
The bacteria Clostridium botulinum causing botulism. Botulism also known as botulinus
intoxication is a rare but serious paralytic illness caused by botulinum toxin, which is produced by the
bacterium Clostridium botulinum.CDP-diacylglycerol-serine O-phosphatidyltransferase is an enzyme
that catalyzes the CDP-diacylglycerol, L-serine and produce Cytidine monophosphate (CMP), (3-snphosphatidyl)-
L-serine in Clostridium botulinum. CMP is a nucleotide that is helpful in RNA synthesis in
Clostridium botulinum.The objective of enzyme inhibition needs structural characterization from its 3-D
structure in rational structure based drug design. Homology modeling can produce high-quality structural
models when the target and templates are closely related. MODELLER is the program used for
homology modeling which provides accurate and efficient models to build loops and side chains found
non-identical in sequence. It implements comparative protein structure modeling by satisfaction of
spatial restraints. The stereo chemical quality of a best model is validated by PROCHECK server with
86.2% residues under favored region from Ramachandran plot. The CASTp is used for locating,
delineating and measuring concave surface regions on three-dimensional structures of proteins. The
residues are identified in burial cavity of the best model as Leu (131,141,156), Ala (132), Ile (138,142),
Asn (145), Thr (149,153), and Gly (151) with hydrophobic nature. These include pocket located on
protein surfaces and voids buried in the interior of proteins that are frequently associated with binding
events. Thus present studies of modeling of CDP-diacylglycerol-serine O-phosphatidyltransferas
enzyme has brought future prospective to fight against botulism disease and provide better health
standaaards for community.