International Journal of Drug Discovery

10-13

Authors: Gomase V.S., Phadnis A.C., Waghmare Somnath

Scorpion venom is an antigenic, water-soluble, heterogenous mixture. The Na+-channel specific toxins from scorpions are modifiers of the channel gating mechanism. The recombinant DNA vaccines involved targeting multiple antigenic components to direct and empower the immune system to protect the host from infection. Limitation of therapy to the treatment of patients suffering from various adverse reaction and contraindications are always experienced. Antigenic epitope on iberiotoxin - eastern Indian scorpion are important determinant of protection against spider venom. As our knowledge of the immune responses to a protein antigen progressed, it became clear that the whole protein is not necessary for raising the immune response, but small segments (DVDCSVSKECWSVCKDLFG; 4-22) of protein called the antigenic determinant or the epitope is sufficient for eliciting the desired immune response. In analysis predicted antigenic epitope iberiotoxin protein is seen. Antigenic epitope from iberiotoxin is a predicted immunization strategy against various diseases.

18-20

Authors: Gomase V.S., Shankar S., Gangawane A.K., Sherkhane A.S.

The toxin LTx5 toxin is an 116aa residue peptide is isolated from the venom of Lasiodora sp. IBSP8539.In this assay we have predicted the binding affinity of toxin LTx5 toxin having 116 amino acids, which shows 109 nonamers. Peptide fragments of the neurotoxin can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in neurotoxin studies. Small segment ‘3- STFIIMISLAVALATWPSEH-22’ of toxin protein called the antigenic epitopes is sufficient for eliciting the desired immune response. Immunization cassettes should be capable of immunizing of broad immunity against both humoral and cellular epitope thus giving vaccines the maximum ability to deal with neurotoxin protein of Eurypelma californicum. Binding ability prediction of antigen peptides to MHC class molecules is important in vaccine development, We also found the SVM based MHCII-IAb peptide regions, 40- YALADRAEK, 10- ISLAVALAT, 99- MYQAERALE, 14- VALATWPSE, (optimal score is 1.494); MHCII-IAd peptide regions, 96- LDIMYQAER, 29- SETKLNVEL, 68- GASVLCEAV, 4- STFIIMISL, (optimal score is 0.604); MHCII-IAg7 peptide regions , 38- GPYALADRA, 98- IMYQAERAL, 9- MISLAVALA, 99- MYQAERALE, (optimal score is 1.904); and MHCII- RT1.B peptide regions, 17- ATWPSEHIE, 101- QAERALEKL, 98- IMYQAERAL, 108- KLASSFRCE, (optimal score is 0.601) which represented predicted binders from neurotoxin protein. We have predicted a successful immunization.