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Effect of royal jelly on in vitro fertilization and early embryo development following nicotine treatment in adult female rats
Authors: Zhila Khodabandeh, Vahid Nejati, Ali Shalizar-Jalali, Gholamreza Najafi, Fatemeh Rahmani
Number of views: 135
Objective: To scrutinize the protective role of royal jelly as an anti-
oxidant on nicotine-induced changes in malondialdehyde (MDA)
level, p53 expression, in vitro fertilization (IVF) rate, and early
embryo development in adult female rats.
Methods: A total of 56 adult female Wistar rats were divided into
8 groups (n=7 in each group). Group 1 served as an untreated
control group, group 2, 3 and 4 received nicotine at a dose of 0.50,
1.00 and 2.00 mg/kg respectively, group 5 received royal jelly at
a dose of 100.00 mg/kg, and group 6, 7 and 8 received 0.50,
1.00 and 2.00 mg/kg nicotine, respectively, with 100.00 mg/kg
body weight royal jelly. Nicotine and royal jelly were administered
daily for 49 days in the experimental groups intra-peritoneally and
orally, respectively. At the end of the experimental period, p53
expression, IVF rate and early embryo development as well as MDA
concentration were measured.
Results: The IVF rate, number of cumulus oocytes, two-cell
embryos and blastocysts decreased in the nicotine-treated groups in
a dose-dependent manner. In addition, p53 mRNA expression and
MDA levels increased in the nicotine-treated groups. Royal jelly
co-administration led to partial improvement in the aforementioned
parameters.
Conclusions: Royal jelly may have a repro-protective effect in
nicotine-administered female rats in terms of its anti-oxidant and
anti-apoptotic properties.