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Effect of Diospyros kaki enriched extender on cattle bull sperm parameters and conception rate
Authors: El-Sheshtawy RI, El-Nattat WS
Number of views: 320
Objective: To explore the effect of Diospyros kaki on cattle spermatozoa during chilling and
cryopreservation. Methods: Five milliliter of blended Persimmon (Diospyros kaki) flesh
was added to 45 mL TCF to obtain 10% stock solution. Kaki enriched extender (KEE) was
prepared by adding to TCF in concentrations 0.0/5.0 mL (control, 0%), 0.5/4.5 mL (1%), 1/4
mL (2%), 1.5/3.5 mL (3%), 2.0/3.0 mL (4%), 2.5/2.5 mL (5%), 3.0/2.0 mL (6%), 3.5/1.5 mL
(7%), 4.0/1.0 mL (8%), 4.5/0.5 mL (9%) and 5.0/0.0 mL (10%) to obtain a final volume 5
mL in each tube. Whole egg yolk was added to each tube to obtain KEE with 20% egg yolk
(KEEY), all tubes were centrifuged to get rid of debris. Semen was added to the supernatants
in other tubes. Extended semen was subjected to evaluation (motility, alive sperm and intact
sperm membrane (HOST) %) in both chilled and cryopreserved semen. Conception rate was
carried out. Results: Sperm motility was significantly (P<0.000 1) kept high after 11 d of
chilling with the concentration 1%, 2%, 3%, 4%, 5% as compared to the control (41.67±1.67,
41.67±1.67, 40.00±0.00, 41.67±1.67 and 41.67±1.67, respectively) and also non-significantly
kept high at the other concentrations up to 9 d of chilling. Addition of KEE had significantly
(P<0.003 3) improved post thawing sperm motility % with the concentrations 1, 2, 3, 4, 5 and
6% as compared to the control (51.67±5.27, 55.00±3.16, 48.33±1.05, 45.00±3.96, 57.00±2.50,
55.00±5.00 and 43.33±5.11 respectively).While the other concentrations exhibit no effect.
Addition of KEE maintained alive sperm%, abnormalities% and % of intact spermatozoa
membranes (HOST %) as good as the control with all concentrations of kaki used in our study.
The conception rate upon using frozen semen in insemination showed higher conception rate in
concentrations of 2%, 4% and 6 % KEE in cattle. Conclusion: It could be concluded that some
concentrations of Diospyros kaki improved bull semen quality post-chilling and post-freezing.