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Maternal reproductive health: Expression patterns of antioxidant enzyme selenoproteins of post-implantation embryos conceived by ethanol-treated murine mothers supplemented with α-tocopherol
Authors: Gliceria B Ramos, Alfonso Noel, Oyong Glen, Sia Aleli Jillian L
Number of views: 387
Objective: To investigate if the protective effect of毩-tocopherol against the impact of
ethanol on brain morphogenesis involved the activity of the selenoproteins phospholipid
hydroperoxide glutathione peroxidase (PHGPx; GPx4) and selenoprotein P (SelPP) that have
roles against oxidative stress. Methods: Forty female mice were randomly assigned into
natural control (CON), positive control (ETOH), low-, medium-, and high-Objective: To investigate if the protective effect of α-tocopherol against the impact of
ethanol on brain morphogenesis involved the activity of the selenoproteins phospholipid
hydroperoxide glutathione peroxidase (PHGPx; GPx4) and selenoprotein P (SelPP) that have
roles against oxidative stress. Methods: Forty female mice were randomly assigned into
natural control (CON), positive control (ETOH), low-, medium-, and high-α-tocopherolsupplemented-
ethanol groups (LTOC, MTOC, HTOC, respectively). CON received drinking
water without ethanol while ETOH, LTOC, MTOC and HTOC groups received 20% ethanol
in drinking water. The supplemented groups were given respective dosages of α-tocopherol,
0.410, 0.819, and 1.640 mg/g body weight, at day 14 before mating onwards to the day 9 of
gestation. At 10.5 ED of gestation (1100 h), the pregnant females were sacrificed by cervical
dislocation and the embryos were harvested. Total RNA were extracted, cDNA synthesis
and qRT- PCR analyses were carried out. Results: The level of expression of PHGPx in the
positive control was significantly lower than that of the natural control. Among the threeα-
tocopherol-supplemented groups, only the medium dose- group was significantly higher than
the positive control. The level of expression of SelPP in the positive control was significantly
lower than those of the natural control, the low- and medium- dose α-tocopherol supplemented
groups. In the high dose-α-tocopherol supplemented group, the level of expression was
not significantly different from the positive control but significantly lower than the natural
control. Conclusions: The activity of the selenoproteins PHGPx and SelPP are involved in the
internetwork of antioxidative enzymes with vitamin E when given up to a medium dose only
and is one of the possible pathways of shielding embryonic development against the impact of
ethanol on brain morphogenesis. This study strengthens the impact of dietaryα-tocopherol and
Selenium supplement during the critical period of pregnancy.Objective: To investigate if the protective effect of α-tocopherol against the impact of
ethanol on brain morphogenesis involved the activity of the selenoproteins phospholipid
hydroperoxide glutathione peroxidase (PHGPx; GPx4) and selenoprotein P (SelPP) that have
roles against oxidative stress. Methods: Forty female mice were randomly assigned into
natural control (CON), positive control (ETOH), low-, medium-, and high-α-tocopherolsupplemented-
ethanol groups (LTOC, MTOC, HTOC, respectively). CON received drinking
water without ethanol while ETOH, LTOC, MTOC and HTOC groups received 20% ethanol
in drinking water. The supplemented groups were given respective dosages of α-tocopherol,
0.410, 0.819, and 1.640 mg/g body weight, at day 14 before mating onwards to the day 9 of
gestation. At 10.5 ED of gestation (1100 h), the pregnant females were sacrificed by cervical
dislocation and the embryos were harvested. Total RNA were extracted, cDNA synthesis
and qRT- PCR analyses were carried out. Results: The level of expression of PHGPx in the
positive control was significantly lower than that of the natural control. Among the threeα-
tocopherol-supplemented groups, only the medium dose- group was significantly higher than
the positive control. The level of expression of SelPP in the positive control was significantly
lower than those of the natural control, the low- and medium- dose α-tocopherol supplemented
groups. In the high dose-α-tocopherol supplemented group, the level of expression was
not significantly different from the positive control but significantly lower than the natural
control. Conclusions: The activity of the selenoproteins PHGPx and SelPP are involved in the
internetwork of antioxidative enzymes with vitamin E when given up to a medium dose only
and is one of the possible pathways of shielding embryonic development against the impact of
ethanol on brain morphogenesis. This study strengthens the impact of dietaryα-tocopherol and
Selenium supplement during the critical period of pregnancy.Objective: To investigate if the protective effect of α-tocopherol against the impact of
ethanol on brain morphogenesis involved the activity of the selenoproteins phospholipid
hydroperoxide glutathione peroxidase (PHGPx; GPx4) and selenoprotein P (SelPP) that have
roles against oxidative stress. Methods: Forty female mice were randomly assigned into
natural control (CON), positive control (ETOH), low-, medium-, and high-α-tocopherolsupplemented-
ethanol groups (LTOC, MTOC, HTOC, respectively). CON received drinking
water without ethanol while ETOH, LTOC, MTOC and HTOC groups received 20% ethanol
in drinking water. The supplemented groups were given respective dosages of α-tocopherol,
0.410, 0.819, and 1.640 mg/g body weight, at day 14 before mating onwards to the day 9 of
gestation. At 10.5 ED of gestation (1100 h), the pregnant females were sacrificed by cervical
dislocation and the embryos were harvested. Total RNA were extracted, cDNA synthesis
and qRT- PCR analyses were carried out. Results: The level of expression of PHGPx in the
positive control was significantly lower than that of the natural control. Among the threeα-
tocopherol-supplemented groups, only the medium dose- group was significantly higher than
the positive control. The level of expression of SelPP in the positive control was significantly
lower than those of the natural control, the low- and medium- dose α-tocopherol supplemented
groups. In the high dose-α-tocopherol supplemented group, the level of expression was
not significantly different from the positive control but significantly lower than the natural
control. Conclusions: The activity of the selenoproteins PHGPx and SelPP are involved in the
internetwork of antioxidative enzymes with vitamin E when given up to a medium dose only
and is one of the possible pathways of shielding embryonic development against the impact of
ethanol on brain morphogenesis. This study strengthens the impact of dietaryα-tocopherol and
Selenium supplement during the critical period of pregnancy.α-tocopherolsupplemented-
ethanol groups (LTOC, MTOC, HTOC, respectively). CON received drinking
water without ethanol while ETOH, LTOC, MTOC and HTOC groups received 20% ethanol
in drinking water. The supplemented groups were given respective dosages of 毩-tocopherol,
0.410, 0.819, and 1.640 mg/g body weight, at day 14 before mating onwards to the day 9 of
gestation. At 10.5 ED of gestation (1100 h), the pregnant females were sacrificed by cervical
dislocation and the embryos were harvested. Total RNA were extracted, cDNA synthesis
and qRT- PCR analyses were carried out. Results: The level of expression of PHGPx in the
positive control was significantly lower than that of the natural control. Among the threeα-
tocopherol-supplemented groups, only the medium dose- group was significantly higher than
the positive control. The level of expression of SelPP in the positive control was significantly
lower than those of the natural control, the low- and medium- dose α-tocopherol supplemented
groups. In the high dose-α-tocopherol supplemented group, the level of expression was
not significantly different from the positive control but significantly lower than the natural
control. Conclusions: The activity of the selenoproteins PHGPx and SelPP are involved in the
internetwork of antioxidative enzymes with vitamin E when given up to a medium dose only
and is one of the possible pathways of shielding embryonic development against the impact of
ethanol on brain morphogenesis. This study strengthens the impact of dietaryα-tocopherol and
Selenium supplement during the critical period of pregnancy.