European Journal of Molecular Biotechnology

Number 3 (13), 2016

94-102
Novel Hemicyanine and Aza-Hemicyanine Dyes: Synthesis, Spectral Investigation and Antimicrobial Evaluation

Authors: H. A. Shindy, A. K. Khalafalla, M. M. Goma, A. H. Eed

Abstract

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Novel hemicyanine dyes and aza-hemicyanine dyes having the nucleus of furo[(3,2-d)pyrazolium; ( )imidazol] iodide salt were prepared. The electronic visible absorption spectra of all the synthesized hemicyanine dyes and aza-hemicyanine dyes were investigated and in 95 % ethanol solution to evaluate their spectral characterization. The antimicrobial effects of some selected dyes were tested against various bacterial and fungal strains (Escherichia coli, Staphylococcus aureus, Aspergillus flavus and Candida albicans) to assess their antimicrobial (bactericidal and fungicidal) properties. The results discussed in this study revealed that both the spectral characterization and the antimicrobial properties of the examined dyes is highly effected by the type of the X substituted in the phenyl ring system for the hemicyanine dyes and by the type of the phenyl and/or the naphthyl ring system for the aza-hemicyanine dyes. Structural confirmations were identified by elemental analysis, visible spectra, IR and 1H NMR spectroscopic data.

103-113
Tissue Engineering Constructs for Osteoarthritis Treatment: a Control of Remodeling

Authors: Alexander A. Shirokiy, Valery V. Novochadov

Abstract

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Tissue engineering and regenerative medicine technologies (TERM technologies) remarkable progress allowed to use predominantly minimally invasive arthroscopic techniques to treat traumas and chronic joint diseases. The essence of joints tissue engineering is development and manufacturing bioengineering matrices (scaffolds) and their following implantation in cell-free variant, or previously populated by suitable cell pool to recover defects by a full-value 3D-structure. The important challenge here is to make individualized scaffolds, which properties are meet the requirements of person and his cartilage defect. The main objective of the study is to describe tissue engineering system “cartilage – scaffold” using systems biology and biocybernetics approaches. The task is to predict development of considered system through time and investigate the possibility to define and solve the control problem which could open the door for property-oriented scaffolds development. Authors explored the referred tissue engineering system as feedback-controlled system. Then we proposed the system of difference equations, which describe its dynamics. Results of computer simulation and forecasted values of extracellular matrix and cells volume ration are in physiological intervals and on a first approximation correspond with previously obtained experimental ones. The next step is to modify model for inverse solution of developing new generation of tissue engineering implants with predefined and controlled characteristics.