European Journal of Molecular Biotechnology

52-59

Authors: N. Ourhriss, A. Zeroual, C. A. Gadhi, A. Benharref, A. Abourriche, A. Bennamara, A. ElHajbi

3,5,5,9-tetramethyl-2-nitro-6,7,8,9-tetrahydro-5H-benzocycloheptene and 2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene has been synthesized using nitration reaction of ar-himachalene. Also, optimized geometry of the title compound are evaluated using HF and DFT/B3LYP with 6-31(d) basis set 6-31G(d) methods. Moreover, the 13C NMR chemical shift values of the molecule (2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene) are calculated using HF and DFT/B3LYP/6-31G(d) and compared with experimental results. The global of the reactivity indices and Parr functions of the reagents were calculated by HF and DFT to identify the actives regions of these molecules, we found that these reactions are regiospecific.

66-73

Authors: Baigalmaa Luvsandorj, Morigen

The Escherichia coli two-component system, QseB/QseC signaling regulates expressions of more than 50 genes encoding flagellar proteins and proteins associated with virulence. In this work, we found that absence of the qseB gene led to an early initiation of chromosomal replication in a manner of medium-independent, the early initiation was shown to be reversed by ectopic expression of QseB from a plasmid pqseB. Further, absence of the qseB gene resulted in an increase of DnaA concentration and the purified QseB protein did not interact with the oriC DNA in vitro. The early initiation was also found in the ∆qseC mutant cells. These results indicate that absence of the QseB/QseC signaling increases concentration of DnaA and the later subsequently triggers the early initiation of replication. Flourescene microscope analysis showed that the QseC protein localized at cell membrane while QseB was in cytosol.

80-88

Authors: Oleg V. Gradov

The monograph (Luisi P.L. "The Emergence of Life: From Chemical Origins to Synthetic Biology", 2010, Cambridge University Press, Cambridge, New York etc., 315 p.) is a well-written, informative book providing a novel view on the interrelation between the abiogenesis as the natural origin of life and synthetic biology as the artificial synthesis of life. This concept is specially known as autopoiesis. As its name implies, it is a correlate of self-organization, but this word has quite a broad meaning in the literature. Consequently, some further restriction is required for this term in abiogenetic, as well as in "biogenetic" applications. There is, in fact, one basic reason for considering the abiogenetic problem in terms of self-organization theory. It follows from the extremely boundless complexity of biological systems.