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Identification of Panton-Valentine leukocidin virulence gene in methicillinresistant Staphylococcus aureus isolated from clinical specimens of burn patients in Zare Hospitals of Sari, Iran
Authors: Atefeh Ranjkesh, Zahra Salari, Mehrab Nejati, Emad Behboudi, Ali Ramezani, Milad Zandi, Mahmood Marzban, Hadis Kiani, Mina Bavi, Mehdi Rabiei Roodsari, Yousef Esvand Zibaee, Samaneh Abbasi, Maryam Mohammadi Elyasi, Mehdi Parsanahad , Shahram Jalilian , Simin Mohammadi Gorji , Mina Owrang
Number of views: 262
Objective: To identify the pathogenic gene of Panton-Valentine
leukocidin in methicillin-resistant Staphylococcus aureus strain
isolated from clinical specimens of burn patients in Shaheed Zare
Burn Hospital.
Methods: A total of 104 samples of Staphylococcus were collected
and 78 aureus samples were isolated from Zare Hospital patients
from November 2016 to July 2017. All isolates were identified
using a standard biochemical and laboratory methodology
in accordance with CLSI principles, and disk agar diffusion
antibiogram were performed to identify methicillin resistant
colonies. Then the presence of the Panton-Valentine leukocidin
gene was tested by PCR.
Results: Of the methicillin-resistant Staphylococcus aureus
samples, 80% were negative for the Panton-Valentine leukocidin
gene, and only 20% of the samples had Panton-Valentine
leukocidin gene. Male and female patients showed no significant
difference in the positivity rate of Panton-Valentine leukocidin
gene (16.12% vs. 33.33%) (P=0.25). Besides, there was no
significant difference in bacterial resistance or susceptibility to
antibiotics between samples with or without the Panton-Valentine
leukocidin gene.
Conclusions: In recent years, increased resistance has been a
serious threat. The resistance or susceptibility of Staphylococcus
aureus strains to different antibiotics is different in different
geographical locations.