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Detection of microRNAs in patients with sepsis
Authors: Michael A. Puskarich, Utsav Nandi, Nathan I. Shapiro, Stephen Trzeciak, Jeffrey A. Kline, Alan E. Jones
Number of views: 494
Objective: To externally validate the diagnostic and prognostic value of three previously
identified microRNAs in emergency department patients with sepsis. Methods: Patients meeting
consensus criteria for sepsis and septic shock were compared to controls. Three microRNAs
(miR-150, miR-146a, and miR-223) were measured using real-time quantitative PCR, and levels
of miRNAs were compared among the three cohorts. The association between miRNAs and both
inflammatory markers and Sequential Organ Failure Assessment (SOFA) score were compared.
To assess the prognostic value of each miRNA, unadjusted and adjusted logistic regression
models were constructed using in-hospital mortality as the dependent variable. Results: Ninetythree patients were enrolled; 24 controls, 29 with sepsis, and 40 with septic shock. We found no
difference in serum plasma miR-146a or miR-223 between cohorts, and found no association
among these microRNAs and either inflammatory markers or SOFA score. miR-150 demonstrated
a significant correlation with SOFA score (ρ= 0.31, P=0.01) and IL-10 (ρ= 0.37, P=0.001), but no
IL-6 or TNF-α (P=0.046, P=0.59). Logistic regression demonstrated miR-150 to be independently
associated with mortality, even after adjusting for SOFA score (P=0.003) or initial lactate (P=0.01).
Conclusions: miR-146a and miR-223 demonstrated no significantly diagnostic or prognostic
ability in this cohort. miR-150 was associated with inflammation, severity of illness, and
mortality. Given the independent predictive value of miR-150, additional research regarding its
role in sepsis is warranted.