Anti-Candida and anti-Cryptococcus evaluation of 15 non-alkaloidal compounds from Pterogyne nitens
Authors: Caroline Sprengel Lima, Carlos Roberto Polaquini, Mariana Bastos dos Santos, Fernanda Patrícia Gullo2, Fernanda Sangalli Leite2, Liliane Scorzoni2, Vanderlan da Silva Bolzani3, Maria Jose Soares Mendes-Giannini, Ana Marisa Fusco-Almeida, Andreia Alves Rezende, Luis Octavio Regasini
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Objective: To evaluate anti-Candida and anti-Cryptococcus activities of 15 nonalkaloidal
compounds from Pterogyne nitens Tulasne (Leguminosae), a South American
Methods: Compounds were submitted to antifungal assays, using microdilution method
described by Clinical and Laboratory Standards Institute document, with minor modifications.
Five species of Candida and two species of Cryptococcus, including clinical
isolates were screened. Antifungal activity was expressed by minimum inhibitory concentration
(MIC). Amphotericin B and fluconazole were used as standard antifungal drugs.
Results: Among tested compounds, six substances presented fungal growth inhibition
(MIC < 31.2 mg/mL) [three flavone derivatives (1–3), a glycosylated flavonol derivative (5)
and two phenolic acids (10 and 12)]. Sorbifolin (1), exhibited potent antifungal activity,
demonstrating MIC value of 3.90 mg/mL against Candida glabrata ATCC 90030, Cryptococcus
gattii 118 and fluconazole-resistant clinical isolate of Cryptococcus neoformans
var. grubii. Pedalin (2) and nitensoside B (3), two glycosylated flavone derivatives, were
active against Cryptococcus neoformans ATCC 90012 (MIC = 7.80 mg/mL).
Conclusions: Flavone derivatives from Pterogyne nitens can serve as prototypes for the
design and development of innovative anti-Candida and anti-Cryptococcus hits.