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Car ies-related factors and bacter ial composition of supragingival plaques in car ies free and car ies active Alger ian adults
Authors: Amina Hoceini, Nihel Klouche Khelil, Ilhem Ben-Yelles, Amine Mesli, Sara Ziouani, Lotfi Ghellai, Nadia Aissaoui, Fatima Nas, Mounia Arab
Number of views: 276
Objective: To compare oral hygiene practices, education and social background, food
intake and oral malodor of Algerian adults suffering from dental caries with normal
controls, and to determine and compare the bacterial composition of the supragingival
plaques from the above-mentioned groups.
Methods: Participants completed a questionnaire and were clinically examined for
dental caries using decayed, missing and filled teeth index according to the criteria laid
down by the World Health Organization. Supragingival plaque samples were collected
from 50 caries-free adults (CF) and 50 caries-active adults (CA). Standard procedures of
culture and identification of aerobic and anaerobic bacteria were used. Data were
analyzed using Chi-square test.
Results: A total of 117 bacterial strains were isolated from supragingival plaques in CF
group subjects, 76 (64.96%) of them belonged to 9 aerobic genera, and 41 (35.04%) to 9
anaerobic genera (P < 0.05). While in the second group, 199 strains were isolated, 119
(59.80%) of the strains belonged to 10 aerobic genera and 80 (40.20%) to 10 anaerobic
bacteria (P < 0.05). Streptococcus mutans, Enterococcus faecium, Aerococcus viridans,
Actinomyces meyeri, Lactobacillus acidophilus and Eubacterium limosum showed a
significantly higher prevalence in the CA group (P < 0.05). The findings revealed that CA
group had a high sugar intake (80%). A significantly higher frequency of tooth brushing
(P < 0.000) and a significantly less self-reported oral malodor (P < 0.000) and tooth pain
(P < 0.000) were found in CF group, while there was no association of socioeconomic
levels and intake of meal snacks with dental caries.
Conclusions: This study confirms the association of some aciduric bacteria with caries
formation, and a direct association of sugar intake and cultural level with dental caries.
Furthermore, oral hygiene practices minimize the prevalence of tooth decay.