Protective effect of Tetracera scandens L. leaf extract against CCl4-induced acute liver injury in rats
Authors: Tung Bui Thanh, Hai Nguyen Thanh, Hue Pham Thi Minh, Huong Le-Thi-Thu, Huong Duong Thi Ly, Loi Vu Duc
Number of views: 229
Objective: To investigate the protective potential of ethanolic extracts of Tetracera scandens L.
(T. scandens) against CCl4 induced oxidative stress in liver tissues.
Methods: Dried leaf powder of T. scandens was extracted with ethanol and concentrated to
yield a dry residue. Rats were administered with 100 mg/kg of ethanolic extracts orally once
daily for one week. Animals were subsequently administered with a single dose of CCl4
(1 mL/kg body weight, intraperitoneal injection). Various assays, such as serum levels of
alanine aminotransferase, aspartate aminotransferase, lipid peroxidation, protein oxidation
(carbonyl protein group), tumor necrosis factor alpha, catalase, superoxide dismutase, and
glutathione peroxidase, were used to assess damage caused by CCl4 and the protective effects
of the ethanol extract on liver tissues.
Results: Hepatotoxicity induced by CCl4 was evidenced by a significant increase in serum
aspartate aminotransferase and alanine aminotransferase level, lipid peroxidation, protein
carbonyl group, and tumor necrosis factor alpha, as well as decreased activity of the hepatic
antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase). Treatment
with ethanolic T. scandens extracts prevented all of these typically observed changes in CCl4-
Conclusions: Our findings indicate that T. scandens has a significant protective effect against
CCl4 induced hepatotoxicity in rat, which may be due to its antioxidant properties.