PPARγ expression by rambutan peel extract in obesity rat model-induced high-calorie diet
Authors: Sri Rahayu Lestari, Muhammad Sasmito Djati, Achmad Rudijanto, Fatchiyah Fatchiyah
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Objective: To monitor the physiological characteristics and genes expression of obesity
rat model after rambutan peel extract (RPE) treatment.
Methods: Twenty-four 12-week-old male rats were divided into 4 groups: normal,
obesity, obesity treated with ellagic acid (O-EA) and obesity treated with RPE30 (ORPE30). Physiological characteristics were monitored by measuring body weight, calorie
intake, size of adipocyte and level of triglyceride. Peroxisome proliferator activated receptor gamma (PPARg), CCAAT/enhancer-binding proteins a and fatty acid binding
protein 4 (FABP4) expression were observed using immunohistochemistry, Western
blotting and quantitative RT-PCR methods.
Results: Body weight gain of O-EA and O-RPE30 rats were lower than obesity group
and size of adipocyte cells were smaller than obesity group (P < 0.05), but when we
compared to normal group, those groups had higher body weight gain and larger
adipocyte cells. The level of triglycerides, protein expression of PPARg and mRNA level
of FABP4 genes were significantly downregulated on O-EA and O-RPE30 compared to
obesity group (P < 0.05). Our results indicated that RPE had potential substance as inhibitor of body weight gain, declining of size of adipocyte, level of triglycerides, PPARg
expression and mRNA level of FABP4 gene on obesity rat model.
Conclusions: RPE have anti-obesity activity by inhibiting body weight gain, declining
size of adipocyte, decreasing triglyceride, PPARg expression and mRNA level of FABP4
gene on obesity rat model.