In vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts
Authors: Nadira Binte Samad, Trishna Debnath, Michael Ye, Md. Abul Hasnat, Beong Ou Lim
Number of views: 347
Objective: To investigate in vitro antioxidant and anti-inflammatory activities of Korean
blueberry (Vaccinium corymbosum L.).
Methods: Total phenolic and flavonoid contents of the Korean blueberry water and ethanol
extracts were determined before determining the potential of the extracts as antioxidant.
Antioxidant activity of the extracts was determined by following some well established methods
r free radical scavenging such as 2,2-diphenyl-picrylhydrazyl hydrate, 1,2,2-azinobis-(3-
ethylbenzothiazoline-6-sulfonicacid), free radical induced DNA damage, superoxide dismutaselike and catalase assay etc. Furthermore, 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan and
nitric oxide assay were performed to determine the anti-inflammatory activity of the extracts.
Results: Total phenolic contents were found (115.0±3.0) and (4.2±3.0) mg GAE/100 g fresh mass for
both extracts, respectively and flavonoid contents were (1 942.8±7.0) and (1 292.1±6.0) mg CE/100g
esh mass for water and ethonal extracts, respectively. Both the extracts displayed significant
scavenging activity of some radicals such as 2,2-diphenyl-picrylhydrazyl hydrate (IC50 at 1.8 mg/
mL and 2.05 mg/mL, respectively), 1,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid) (IC50 at
1.5 mg/mL and 1.6 mg/mL, respectively) and nitrite (IC50 at 1.7 mg/mL and 1.5 mg/mL, respectively)
etc. The extracts were found to prevent inflammation as well by reducing nitric oxide production
and cytotoxicity in cell.
Conclusions: The findings suggest that the fresh Korean blueberry could be used as a source of
natural antioxidants and anti-inflammatory agents.