443-447
Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf
Authors: Mahendra Jain, Rakhee Kapadia, Ravirajsinh N Jadeja, Menaka C Thounaojam, Ranjitsinh V Devkar, SH Mishra
Number of views: 316
Objective: To evaluate the cytotoxicity and hepatoprotective potentials of extracts, fractions
or isolated compound from the leaves of Feronia limonia (F. limonia). Methods: Qualitative
phytochemical analysis of extracts, fractions or compound was performed by means of thin layer
chromatography and spectroscopic assays. The % purity of compound was measured by analytical
HPLC. Extracts, fractions or compound have been individually evaluated for their cytotoxicity
effects (10, 20, 100, 250, 500, 750 and 1 000 µg/mL). Based on the inhibitory concentration (IC50)
obtained from the cell viability assay, graded concentrations of extracts, fractions or isolated
compound were assessed (10, 20, 50, 100, 200 µg/mL) for its hepatoprotective potential against
CCl4-induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic
transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT). Results: Results
indicated that the methanol extract of F. limonia was non-toxic and hepatoprotective in nature
as compared with the petroleum ether extract. The acetone fraction of methanolic extract also
showed similar properties but the subsequent two fractions were cytotoxic. However, the pure
compound isolated from the penultimate fraction of methanolic extract was non-toxic and
hepatoprotective in nature. Biochemical investigations (SGOT, SGPT) further corroborated these
cytological observations. Conclusions: It can be concluded from this study that F. limonia
methanol extract, some fractions and pure isolated compound herein exhibit hepatoprotective
activity. However, cytotoxicity recorded in the penultimate fraction and investigation of structural
details of pure compound warrants further study.