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Вміст загальних ліпідів і співвідношення їх фракцій у тканинах грудей і черевця медоносних бджіл за згодовування борошна з бобів нативної та генетично модифікованої сої
Authors: Романів Л. І., Федорук Р. С.
Number of views: 370
Influence of compounds of soybean of
native and genetically modified sorts has been
studied at the conditions of feeding in a spring
period on the reproductive function of mothers of
bee families, and also on the content of lipids and
their factions in tissues of breast and abdominal
departments of body of bees. The investigation has
been conducted in spring and summer period on
the 3 groups of bee families of Carpathian breed of
melliferous bees, for 3 families in a group.
A control group has been maintained at stationary
conditions with the envisaged removal of bee-
bread storages 1 time a week. Bees of II
experimental group got in the additional feeding a
meal of native sort of soybean in an amount 200
g/week/familie, and the bees of III experimental
group were fed up by the meal of genetically
modified sort of soybean in an amount
200 g/week/familie. The investigation lasted 35
days with the interval of the additional feeding of 7
days. Families have been formed of 6 frames of
young reproductive mothers, and groups have been
selected by the method of selection of pairs-
analogues. Control and experience groups of
families of bees were safe in relation to infections
and invasions. At the finishing stage of an
experiment the samples of tissues of melliferous
bees have been taken. The content of total lipids
and correlation of their separate factions in the
experimental samples of tissues of melliferous bees
have been determined. The determination of
content of total lipids has been conducted by the
method of lipid extraction by Folch and
correlation of content of separate factions of lipids
by the method of TLC with the use of plates with
Sorbfil PТLC-P-А. Among total lipids in the breath
department of body of melliferous bees of control,
II and III experimental groups have been
discovered: phosphatides — 18,91;
21,61 (р<0,001); 21,09 (р<0,001); mono- and
diacylglycerols — 22,03; 14,70 (р<0,001); 15,62
(р<0,001); free cholesterol — 18,57; 16,24
(р<0,001); 14,09 (р<0,001); free fatty acids —
14,81; 15,44 (р<0,05); 16,64 (р<0,01);
triacylglycerols — 14,37; 19,43 (р<0,001); 18,66
(р<0,001); cholesterol ethers — 11,06; 12,52
(р<0,01); 13,85 (р<0,001). In tissues of abdominal
department of body of control bees, II and III
experimental groups such changes of content of
separate factions of lipids have been shown:
phosphatides — 15,01; 22,24 (р<0,001); 22,01
(р<0,001); mono- and diacylglycerols — 17,66;
14,30 (р<0,001); 12,49 (р<0,001); free cholesterol
— 21,10; 13,67 (р<0,001); 15,44 (р<0,001); free
fatty acids — 16,66; 14,36 (р<0,001);
14,05 (р<0,001); triacylglycerols — 12,93; 15,86
(р<0,001); 17,37 (p<0,001); cholesterol ethers —
16,36; 19,57 (р<0,001);18,59 (р<0,01). The
content of phosphatides, free fatty acids,
triacylglycerols and cholesterol ethers increased in
tissues of breast department of body of melliferous
bees of II experimental group. The content mono-
and diacylglycerols, free cholesterol reliably
decreased. The same increase of content of
separate factions of lipids has been observed in
muscular tissue of breasts of III experimental
group, that got in the additional feeding by meal
from the bobs of genetically modified sort of
soybean. The content of phosphatides,
triacylglycerols and cholesterol ethers increased in
the abdominal department of body of melliferous
bees of II and III experimental groups as compared
to control group. These data can suggest the
positive influence of separate compounds of
soybean meal on metabolism of total lipids and
their separate fractions in tissues of organism of
melliferous bees and on level of exchange of it
structural elements in the process of life and
performance.
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