The Journal of Middle East and North Africa Sciences

1-4

Authors: Catalina Velásquez Sánchez; Jorge Luis Castañeda Gutiérrez; Rosa Cortés Aguirre

Today, security is a very important issue for the global economy, especially in freight transport companies, due to the impact that crime has had lately, and that has led to initiatives to increase security in the chain of supply, especially during the physical movement of the merchandise, taking measures and control actions to increase the effectiveness and effectiveness of the security guidelines, measures that allow them to provide a safe and competitive service. That is why the present research work has the purpose of designing a location and tracking system for transport units for security through GPRS / GSM technologies, the information analyzed to determine the types of goods, the characteristics of the system, was through a field of investigation within the company ZEEK, a branch located in the Municipality of Santa Ana Chiautempan, Tlaxcala, Mexico. In order to determine the type of companies that prefer technologies and invest in equipment, this is done through a Pareto analysis. The feasibility of applying a location and security system has several functions, for example, it helps us in the event of an unwanted event such as door opening, shock or the variation of the load temperature, it increasingly becomes a You need to know the exact location, either for security in case of theft or for control and monitoring, giving it an added value with the implementation of a mobile application that shows the exact location in real time. It was also possible to obtain results through the customers´ information who have the GPS system. During the analysis of the investigation I concluded that it is feasible and necessary to implement a security system based on the need for the care of the goods and thus also safeguard the integrity of the users.

10-14

Authors: Sehrish Ather; Romana Ayub; Adeela Mustafa; Zakria Tariq; Muhammad Saleem Rana

Background: Plasmodium knowlesi is a simian malaria parasite that causes malaria in human also. P. knowlesi detected in human population of many South East Asian countries. It causes life threatening malaria complications. Early detection is very crucial for successful clinical management of knowlesi malaria. Aim: To provide insight into the detection methods used for diagnosis of knowlesi malaria. Method: Detailed reviews of the previous studies on malaria caused by Plasmodium knowlesi with the particular emphasis on diagnostic methods, especially polymerase chain reaction-based molecular detection methods and loop mediated isothermal amplification technique (LAMP) was performed. Result: Due to the morphological similarities between P. knowlesi and other malaria parasites infecting humans, blood film microscopy is not a reliable method for the detection of P. knowlesi. Rapid diagnostic methods based on P. knowlesi-specific antibodies have not been available yet. For half a decade, Nested PCR is in use as the standard method for identification of P. knowlesi. While requiring sophisticated equipment, real-time PCR provides rapid results with higher specificity and sensitivity, so it used in reference laboratories. Loop-mediated isothermal amplification is a promising method for diagnosis of knowlesi malaria in the field. These methods detect small subunit ribosomal RNA as the molecular target. However, recent studies demonstrated the use of multicity, repetitive sequence as the molecular target in single-step PCR detection of P. knowlesi. Conclusion: P. knowlesi misguided for P. malariae by microscopy, which is responsible for high fatalities due to P. knowlesi infections because P. knowlesi produces more severe malaria as compared to P. malariae. Although molecular diagnostic methods are sensitive and specific for P. knowlesi, but these methods are not reliable as compared to gold standard PCR Technique.