Central European Journal of Botany

7-11

Authors: Ömer Kılıç

Tulipa julia is an ornamental important plant and a wide geographical distribution eastern of Turkey. However, due to habitat loss and illegal over collection in the wild it is included as a vulnerable species. The development of a protocol for Tulipa julia bulblet propagation in vitro may be useful for reintroducing plants in their natural habitats, and for germplasm conservation. A difficult problem encountered during the establishment of an in vitro culture is explants disinfection, especially when working with endangered species, from which explant availability is restricted. Thus, the establishment of a sterilization protocol is crucial for the initiation and success of bulblets micropropagation system for Tulipa julia. This study was to evaluate the effect of sodium hypochlorite concentrations and treatments time in bulbs surface disinfection, tissue sensitivity and development. Sodium hypochlorite solutions (2 or 3 %, 20 or 25 min; 4 or 5 %, 30 or 35 min) were effective in eliminating bulbs superficial contaminants. There was significant difference among the effective sterilization sodium hypochlorite concentrations and treatments time in relation to surface sterilization bulbs of Tulipa julia. Also, no damage to bulbs tissues were observed. Surface sterilization of bulbs, for initiation of an in vitro culture, required higher concentrations of sodium hypochlorite (4 or 5 % NaCl, 30 or 35 min) for controlling fungal and yeast contamination, compared to bulbs sterilization.

21-27

Authors: Avni Öztürk a , *, Ömer Kılıç

With plant samples related to Veronica anagolloides Guss. which was previously identified as Veronica michauxii Lam. by Öztürk and mentioned it’s name in his doctoral theises and in a publication. Samples published by M.A.Fischer as Veronica anagolloides Guss. subsp. heureka M.A. Fischer, due to reasons stated in this article it needs to be raised to species level named V. heureka (M.A. Fischer ex) A.Öztürk & Ö.Kılıç. In an article cited samples were given abortive named as V. heureka by Öztürk & Fischer. Because of the impossibilities in 1977, Öztürk identified Veronica anagolloides as Veronica michauxii due to more morphological similarities. M.A. Fischer in his paper which published in 1981, because of the large number of transitional forms in terms of morphological characters and at the time of his impossibilities he had to classify at subpecies level named Veronica anagolloides subsp. heureka. In later years, karyological evidences were obtained and publications were made to support this classification. Their details are mentioned in this article text.