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Irregular virulence genes expression of Vibrio parahaemolyticus in shrimp or seawater matrix
Authors: Haiquan Liu
Number of views: 451
The three virulence genes (tlh, tdh and trh) expression of five Vibrio parahaemolyticus strains (ATCC33847, ATCC17802, VP1, VP2 and VP3) in shrimp or seawater matrix at 9°C and 25°C were detected by the quantitative reverse transcription-PCR (qRT-PCR). The results showed that the transcription levels of tlh in ATCC17802 and VP3, tdh in VP1 were up-regulated, but the transcription levels of tlh and tdh in ATCC33847 were significantly (p < 0.05) down-regulated in shrimp samples compared with that in seawater samples. Additionally, lowtemperature had a positive effect on genes expression, including tlh and tdh in ATCC33847, trh in ATCC17802, tlh in VP2. Meanwhile, lowtemperature had a negative effect on the expression of tlh in ATCC17802 and tdh in VP1. Interestingly, the transcription levels of tdh in VP1 and tlh in VP3 cultured at 9°C and 25°C, respectively, were significantly (p < 0.05) higher in shrimp samples than that in other samples. These results indicated the expression of virulence genes in V. parahaemolyticus were irregular with the varying of environmental conditions.