10
Biocompatibility test with Artemia salinafor five materials for endodontic use
Authors: Leidy Johanna Torres-Avirama, Daniela Alexandra Realpe-Urbano, Tania Lizeth Guevara-Valencia, Gilbert Alfonso Morales, Ingrid Ximena Zamora-Córdoba, Carlos Humberto Valencia-Llano
Number of views: 7
Introduction:Biocompatibility is a decisive factor in the success of endodontic therapy. Objective:To assess the cytotoxicity of five endodontic materials using the Artemia salinatest. Method:An in vitro experimental study was designed, using Artemia salina,to evaluate the biocompatibility of Grossman, CaOH, AH-Plus, MTA, and TheraCal-LC cements, with observations at 24 and 48 hours. Results:Grossman cement presented100% toxicity at 24 hours, while the other materials show values lower than 30%, reflecting a biocompatible behavior; however, at 48 hours, CaOH and TheraCal-LC present toxicity higher than 30%, while AH-Plus and MTA continue with values lower than 30%. The data were analyzed with a Generalized Linear Model with binomial error distribution, a deviancy analysis, and Fisher's multiple comparison test with Bonferroni correction. An independent analysis was performed for 24 and 48 hours, under a significance level α=5%; significant differences were found at 48 hours between TheraCal-LC and AH-Plus, and MTA. Conclusions:Grossman cement presented a high degree of toxicity, while AH-Plus and MTA showed the highest biocompatibility.