Journal of World's Poultry Research

175-179

Authors: Safiullin RT, Safiullin RR and Kachanova EO.

Favorable conditions for development, reproduction, and accumulation of large amounts of zoophilous flies in commercial poultry farms are caused by incomplete compliance with veterinary and sanitary rules for growing in cage facilities. The purpose of the study was to test a systematic insecticidal program for destroying flies’ populations using adulticide and larvicide drugs in poultry farms under battery cage management. The number of imago flies in hen houses was dynamically evaluated using flypapers, six flypapers in each hen house, situated in different levels above the floor. Flypapers were removed and the number of stuck insects was counted. The number of larvae was evaluated in dynamics by specimen testing from the floor area 10x10 cm, with weight of 3-5 g. The Quick Bayt WG 10% was applied to destroy the imago of flies. Baycidal® WP 25% was used against larvae of flies. Complex insecticide program Quick Bayt WG 10% + Baycidal® WP 25% provided the opportunity to destroy flies, with a significant difference in intensefficacy, (98.3 % for adult flies and 99.8 % for larvae). Furthermore, this program had a positive impact on economic indicators of meat production of broilers. The present study demonstrated high preventive efficacy and economical efficacy of complex program against flies under battery cage broiler management.

187-195

Authors: Handayani UF, Wizna, Suliansyah I, Rizal Y and Mahata ME.

Lycopene is a powerful antioxidant present in tomatoes and other vegetables and fruits. Present research was carried out to evaluate lycopene and nitrogen retention and crude fiber (CF) digestibility of steaming tomatoes waste powder which was combined with oil. Tomatoes waste in this experiment were local fresh tomato rejected from tomato field around West Sumatera province, Indonesia. The experimental factors were included the type of oil (palm and coconut oils) and dosage of oils (0.25, 0.5, 0.75, 1, and 1.25 %), and each treatment was replicated three times. The results indicated there was an interaction between the type of oil and dosage of oil on lycopene retention, and CF digestibility, while the type of oil and dosage of oil affected lycopene retention significantly. The dosage of oil also influenced lycopene retention, nitrogen retention, CF digestibility significantly. The addition of coconut oil in steaming tomato waste powder increased lycopene and nitrogen retention, and CF digestibility higher than the addition of palm oil to steaming tomato waste powder in broiler chickens. The lycopene and nitrogen retention, and CF digestibility of steaming tomato waste powder added 0.5% coconut oil was the best level for lycopene and nitrogen retention, and CF digestibility in broiler chickens.

204-210

Authors: Gad S, El-Shazly MA, Wasfy KA and Awny A.

The main purpose of the present study was to find an alternative source for traditional energy to provide the energy requirements in the poultry industry. The present study was conducted in four poultry houses with different heating systems (solar and conventional) and ventilation rates located in El-Sharkia Governorate, Egypt, during June and July 2018. In this study, it was found that productivity increased by increasing the ventilation rate, where productivity reached 2.3 kg when using a solar heating system with a ventilation rate every two minutes. Productivity decreased in poultry houses with a conventional heating system and was 2 kg in ventilation rate every 2 minutes, and 1.8 kg in the ventilation rate every four minutes. The level of ammonia was also reduced with the ventilation rate every two minutes. Concentrations of ammonia ranged from 22 ppm at ventilation rate every two minutes to 28 ppm at the ventilation rate every four minutes. In addition, solar energy provided good levels of thermal requirements. It was demonstrated that solar energy as an alternative source to the conventional energy, is very efficient and can be applied on a large scale when combined with conventional electricity as a light source and within specified limits.

217-223

Authors: Tsega KT, Maina JK and Tesema NB.

Poultry production is presently the most effective animal production industry and provides an excellent source of protein production worldwide. The poultry gastrointestinal microbiota includes commensal, mutualistic and pathogenic microbes. The relationship between host and gut microbiota can affect the balance of mutualism and pathogenicity. The imbalanced gut microflora caused by the incidence of disease, hygiene conditions, diet, management practices, and environmental stress affects the survival and productivity of chicken. Maintenance of the gut microbial composition is possible through the regulation of the gastrointestinal microbiota by suppressing the growth of pathogens. For many years, antibiotic growth promoters have been used to manage these problems. Nowadays, because of the emergence of antibiotic-resistant bacteria, other alternatives are being sought. Supplementation of probiotics as feed additives is considered to enhance chicken productivitity and to protect the gut from pathogen colonization and help to tolerate environmental stress. The goal of the present article was to review the poultry gastrointestinal microflora and probiotics role in the health and growth of poultry. In addition, this article focused on probiotic microorganisms and their potential characteristics.

233-239

Authors: Dukhnytskyi V, Bazaka G, Sokolyuk V, Boiko P and Ligomina I.

The current study was aimed to investigate the effects of feeding Mospilan and Actara insecticides on egg production performance and meat quality of laying hens. Experimental research was conducted in the laboratory of the Department of Pharmacology and Toxicology of the National University of Life and Environmental Sciences of Ukraine in 2015. The experiments were performed on five groups each consisting of seven chickens. The age of the chickens at the beginning of the experiment was 150 days. The birds were fed the granulated compound feed. In M1 and M2 groups, Mospilan at doses of 65 mg/kg and 32.5 mg/kg of body weight were added to the feed, respectively. In A1 and A2 groups, Actara at doses of 360 mg/kg and 180 mg/kg of body weight were added to the feed, respectively. Chickens of the control group were fed without the addition of insecticides to the feed. The feeding period lasted 30 days and finally, egg production performance, meat quality, and gross pathological changes were evaluated. Egg production rate in M1 and M2 groups in comparison to the control group decreased by 78.4 and 29.7%, respectively. Egg production rate in A1 and A2 groups reduced by 89.2% and 48.7% compared to the control group, respectively. Chickens in groups of receiving insecticides had pale skin and enlarged heart, also showed spot hemorrhages in mucous membranes of the glandular stomach and intestine, color heterogeneity of the lungs, and the liver was dark cherry in color with hemorrhage. In addition, the relative weights of internal organs decreased by 23-36% in experimental groups. In the experimental groups, the pH of meat decreased at day 4 post-slaughter, and the meat broth with the addition of 5% copper sulfate solution was slightly cloudy with flakes. The meat of birds from the experimental groups was low toxic. Extracts from chicken meat of the experimental groups caused pathological changes, inhibition of movements and death of 13-16% of Tetrahymena pyriformis infusoria. This study demonstrated that the presence of Mospilan and Aktara in feed reduced the egg production rate, caused chronic poisoning, changed biochemical processes in chicken meat and increased its toxicity.

124-132

Authors: Kongpechr S, Sohsuebngarm D and Sukon P.

Longitudinal bone growth is essential to support rapid body growth in commercial broiler chickens. The present study aimed to determine which simple mathematic model is best suitable for explaining the absolute and the relative bone growth in length (expressed as a ratio of bone length to body weight) as a function of age in male and female commercial broiler chickens over the first 35 days of age. A total of 1,800 broiler chickens (900 males and 900 females) of Cobb 500, Ross 308, and Arbor Acres raised in standard commercial broiler houses were randomly selected for this study. Body weight and the lengths of backbones, third toe, shank bone, and keel bone were individually measured in all chickens at 1, 7, 14, 21, 28, and 35 days of age. Regression analysis (with 4 simple mathematical models including linear, logarithmic, inverse, and exponential) was used to find a suitable model for estimating the absolute and the relative bone growth in length. In addition, an adjusted R2 was used to assess the model fitting. The results indicated that the absolute bone growth in length linearly increased with age. The adjusted R2 values for the linear model were 0.973, 0.937, 0.950, and 0.974 for the lengths of the backbones, third toe, shank bone, and keel bone, respectively. However, the relative bone growth in length logarithmically decreased with age. The adjusted R2 values of the logarithmic model were 0.971, 0.952, 0.957, and 0.905 for the relative length of the backbones, third toe, shank bone, and keel bone, respectively.The present investigation suggests thata linear model is a suitable model for estimating the absolute bone growth in length, but a logarithmic model is a proper model for estimating the relative bone growth in length of commercial broiler chickens.

139-146

Authors: Anggraini L, Marlida Y, Wizna W, Jamsari J and Mirzah M.

Nutrition is an essential factor for microorganisms to grow and survive. Carbon and nitrogen sources are used in producing primary and secondary metabolites. Gamma-Aminobutyric acid (GABA), a non-coded amino acid, is a secondary metabolite which acts as an inhibitory neurotransmitter of the central nervous system. Pediococcus acidilactici DS15 is a bacterium belonging to the order of Lactic Acid Bacteria. This study aimed to determine the effects of nutrients including glutamate, nitrogen and carbon sources on GABA production by Pediococcus acidilactici DS15. The tests were carried out using a range of 30 mM, 40 mM, 50 mM, 60 mM, and 70 mM glutamate as inducer and carbon sources in the form of peptone, yeast extract, skim milk, NH4NO3, KNO3, whey tofu and soy milk as nitrogen sources, and then glucose, lactose, maltosa, sucrose, palm, and cane sugar as carbon sources. The best sources of both will be tested with levels 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100% for nitrogen and 1, 3, 5, 7, 9, 11, 13 and 15% for carbon. The results of this investigation revealed that the addition of 60 mM glutamate caused the higher amount of GABA production and the best source of nitrogen and carbon for Pediococcus acidilactici DS15 were 100% whey tofu and 15% palm sugar, respectively. Production rate of GABA by Pediococcus acidilactici DS15 could reach up to 311,485 mg / L.

154-159

Authors: Maslami V, Mirnawati, Jamsari, Nur YSh and Marlida Y

Feed additives are ingredients that are added to the ration as growth promoters and enhancement of the immune system. Glutamate is a feed additive that improves performance by improving the quality of the small intestine and enhancing the immune system. The purpose of this study was to know the effect of including glutamate as a feed additive in improving broiler performance. The material used in this study was broiler strain MB 202 from PT Charoen Phokphand Indonesia as many as 240 birds. The design used was a Completely Randomized Design (CRD) trial design, with six treatments and four replications, so that there were 24 cage plots as experimental units. Each experimental unit consisted of 10 chickens. The Glutamate doses in groups were, A (0.4% commercial glutamate; B (0% glutamate); C (0.2% glutamate); D (0.4% glutamate); E (0.6% glutamate); F (0.8% glutamate). The results indicated that glutamate up to 0.8% had significant effects on feed intake body weight gain and feed conversion ratio (1.70%), but it did not affect the percentage of carcass. It is concluded that including the 0.8% glutamate in broiler disets can improve broiler performance with 35 days of maintenance.

38-49

Authors: Mulatu K, Ameha N and Girma M.

The effects of feeding baker’s yeast performance of Cobb 500 broilers were studied. Four nearly isocaloric and isonitrogenous starter and finisher rations were prepared. 240 chicks with an average initial body weight of 42g were randomly divided into 12 groups contained 4 treatments with 3 replications for each treatment. Treatment rations were containing 0, 0.5, 1.5 and 2.5% of baker’s yeast as T1, T2, T3 and T4 respectively. At the end of the trial, 3 males and 3 female chickens from each replication were slaughtered for carcass evaluation. The Crude Protein (CP) and Metabolizable Energy (ME) contents of baker’s yeast were 48% and 3615 kcal/kg DM, respectively. The CP content of the rations during the starter and finisher phases were 22% and 20%, respectively. The ME content of the rations during the starter and finisher phases were 3100 kcal/kg and 3200 kcal/kg respectively. Feed intake during the starter phase and entire trial period was lower for T4, whereas during the finisher phase in control diet group showed the highest feed intake than the other supplemental groups. The highest daily body weight gain was recorded in broilers fed T4 rations during starter phase, finisher phase and entire experimental period. Feed conversion ratio of T4 and T3 groups was better than T2 and T1. T3 and T4 groups had higher eviscerated percentages. Blood parameters results showed that fed broilers yeast containing ration had higher WBC, PCV and Hb. Partial budget analysis indicated that the highest net income, marginal rate of return and chicks’ sale to feed cost were obtained for T3 followed by T4. Baker’s yeast can be an important feed additive, which can be included up to 2.5% of the total ration and improve the overall performance of broilers without compromising the hematological indices of broiler chickens.

59-67

Authors: Ouennes H, Afri Bouzebda F, Bouzebda Z, Majdoub S, Djaout A and Adnane Smadi M.

The present aimed to investigate for the first time the characteristics and conservation of post-mortem sperm, obtained from epididymis and the vas deferens of 18 pairs of adult local chicken cocks from the east of Algeria (age, 12-24 months, body weight 1.50-2.53 kg). And compare the sperm quality at the epididymis and the vas deferens levels, the efficacy of two post-mortem sperm retrieval techniques, the flushing and float-out methods in the collection of local chicken cock’s sperm, and the effects of conservation in situ at different temperatures (2h and 24h at 20°C and after refrigeration at 4°C for 24h). The quality was significantly higher (0.13±0.05ml vs 0.72±0.12ml) in the vas deferens compared to the epididymis, whereas spermatozoa abnormalities and chromatin quality did not differ in both the epididymis and vas deferens. No significant difference was observed between the flushing and float-out methods. Concentration (3.33±1.63 million sperm vs 1.75±0.76 million sperm), initial motility (77.50±6.89% vs 75.83±8.61%), viability (75±10.39% vs 74.67±10.15%) and abnormality (30.33±4.68% vs 30.33±4.68%), only the volume was significantly higher (0.72±0.12ml vs 0.17±0.08ml). However, the effects of conservation in situ, at 25°C and 4°C for 24h, showed a significant difference for viability and motility of the spermatozoa recovered from vas deferens, and no significant difference for abnormality and acrosome integrity. Therefore, it can be concluded that good quality semen samples can be collected from the vas deferens with the flushing method, and semen of Algerian local cocks can be preserved at 20°C for 24h.

78-81

Authors: Igenbayev A, Nurgazezova A, Okuskhanova E, Rebezov Y, Kassymov S, Nurymkhan G, Tazeddinova D, Mironova I and Rebezov M.

This paper aimed to study the fatty acid composition of turkey meat. Red and white turkey meat were sampled from the local markets of Semey city, republic of Kazakhstan. The proximate composition showed a significant difference in the fat content of red and white meat. The fatty acid composition of turkey meat was as follows: saturated fatty acids 50.67% in white and 52.64% in red meat; monounsaturated fatty acids 28.07% in white and 23.79% in red meat; polyunsaturated fatty acids 21.26% in white and 23.57% in red meat. Palmitic and pentadecanoic are the major saturated fatty acids, where the oleic and linoleic acids are in a large amount in monounsaturated and polyunsaturated fatty acids, respectively.

89-97

Authors: Berghiche A, Khenenou T and Labiad I.

Present study consisted of performing a meta-analysis on data about the detection of antibiotic residues in chicken meat achieved from all over the researches with a wide collection and very strict selection criteria of data. The databases were searched quantitative inputs from the available scientific publications using important keywords, in order to evaluate all studies about antibiotic residue and detection methods and the reliability of the results obtained by the international researchers. Then an advanced statistical analysis on collected data was done, the first phase was a descriptive study of positive and negative cases followed by a modeling of two cases with a prediction of the values obtained and ended with an analysis of the main compounds (population size, residue detection methods and positive case rates). All performed steps are reported in detail. The results indicated that the accuracy of the detection technique is a factor that influenced on reports of residues, and caused differences in reports, there are still antibiotic residues in meat of intensively broiler chicken farms (45. 26% of the samples analysed are positive), It is concluded that residue detection requires a high-precision qualitative analysis protocol.

109-116

Authors: El-Sergany E, Hamed E-H, El-Sawy H, Medhat T, Yasser A and Alaa E-M.

Clostridium perfringens is the most important cause of enteritis in domestic animals, in chicken and turkey it well known as pathogen responsible for necrotic enteritis; hepatitis, and cholecystitis. The disease in turkey characterize by either severe form with high rate of mortalities or subclinical form of reduce growth rate and increase condemnation rate. The major factor responsible for pathogenicity of Clostridium perfringens was alpha toxin. The aim of present study was to prepare of Clostridium perfringens alpha Toxoid vaccine for controlling the necrotic enteritis disease. The vaccine was prepared at different doses depend on lethality of toxin (24, 48 and 96 Minimum Lethal Dose) for controlling necrotic enteritis disease. Antibody titer elicited by vaccination was measured by toxin neutralization test, ELISA, and challenge test. It revealed that antibody titer expressed by international antitoxin unit per ml was 7.4, 4.1 and 1.26 respectively according to the mentioned dose, and also the protection percent against challenge was 100% when vaccinated with either 48 or 96 Minimum Lethal Dose, while it gave 80% when vaccinated with 24 Minimum Lethal Dose.It concluded that use of Clostridium perfringens alpha Toxoid with recommended dose of 48 MLD able to protect turkey for 6 months.

07-14

Authors: Onyekwereh TO, Adejumo DO, Afolabi KD, Nworgu FCh and Olubisi AO.

Haematological, serum biochemical and haematological histological responses were studied in cockerels undergoing a long-term supplementation with Telfairia occidentalis Leave Extract (TOLE). Haematological and serum biochemical parameters investigated included haemoblobin, white blood cells, red blood cells, lymphocytes, heterophils, monocytes, eosinophils, total protein, aspartate aminotransferase, aspartate amino transferase and triglyceride. Histological changes associated with Telfairia occidentalis leaves extract on the kidney, liver, spleen and testes were also examined. Result showed that birds on TOLE has higher values for most of the haematological parameters studied which were significantly (P < 0.05) higher than the control. Also the total protein, globulin and alanine aminotransferase were significant (P < 0.05) for birds on TOLE having higher values while for triglycerides birds on the control treatment had the higher values which was significant. There were no significant changes in the albumin and aspayrtate aminotransferase. Histological changes showed mild to severe congestion in the spleen and testes of birds that received 120 and 150mL TOLE/L of water that also showed reduced germinal epithelium height and sloughing of the germinal epithelium respectively. Long term supplementation of TOLE for cockerel production should not exceed 60mL of TOLE per liter of water as the administration in excess of this can bring about tissue breakdown and reduced fertility. Animals suffering from blood loss can benefit from the administration of fluted pumpkin leaves extract as the extract increased erythron production.

24-31

Authors: Gulmez M, Gulmez N, Bingol S, Deprem T and Koral Tasci S.

Probiotics are currently under investing the most valuable substances alternative to antibiotic growth promoters in poultry breeding practice. This research was performed to evaluate the effect of supplementing broiler drinking water with probiotics (Pediococcus acidilactici and Bacillus subtilis) at a concentration of ≥108 CFU/ml during 42 days of feeding period on growth performance and gut health. A total of 144 one-day-old Ross 308 broiler chicks (mixed gender) with an average initial BW of 42.3 g were used. The chicks were allotted to pens with 12 birds per pen and six replications per treatment with food and water provided ad libitum. Feed intake of Probiotic group was 4134 g, 338 g less than that of control group. Live weight of probiotic group was 2537 g and a 113 g more than that of control group. The feed conversion ratio of probiotic group was 1.61, 0.22 less than that of control group. The crypt depth of probiotic group (1110.46 ± 224.016 µm) was statistically deeper than that of control group (949.39 ± 114.166 µm) in ileum. Continuously use of probiotics in drinking water of commercial poultry flocks appears to be alternative to AGPs. The results of this study provide a greater understanding of the impact of long-life use of probiotics on broiler health and growth performances.