Journal of Applied Tissue Engineering

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Authors: Mohammad Reza Nourani

Tissue engineering is an interdisciplinary field, involving biology, medicine, and engineering to improve the quality of life in many patients that suffering from different tissue damage in worldwide. The origin of “tissue engineering” as it is documented today can be clearly followed to a definite individual. In 1985, Y.C. Fung, a pioneer of the field of biomechanics and of bioengineering more broadly, submitted a proposal to NSF for an Engineering Research Center to be entitled “Center for the Engineering of LivingTissues.

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Authors: Maboud AlizadehNodeh, Zahra Moradi, Mohammad Reza Nourani

Collagen is one of the main biomaterials with tissue engineering application. It plays an important role in designing and providing scaffold to support the interactions between cells and scaffolds aiming for high attachment, proliferation and differentiation. Currently research proceed to extraction collagen from the skin Black pomfret (Parastromateusniger), one of the most important and economical species of the Oman sea and Persian gulf. Collagen is the most abundant protein in vertebrates; have a biomedical matters, pharmacutical, tissue engineering, industrial and biotechnological application. Samples taken from the fishery market and carried out to laboratory. Acid soluble collagen (ASC) isolated by acidic methods at the 4℃ for 5 day. Then, collagen precipitates with NaCl and purification to 0.1M acetic acid solution and distilled water. The results showed that collagen this fish is highest value of 13.6% on a dry weight basis . The SDS-PAGE pattern show the ASC producted from this species have hetrotrimer structure and made of two chains; α1and α2 . So collagen provides is considered type I collagen. Moreover UV-spectra exhibited this collagen have a absorbance 220nm. Therefore black pomfret fish can be use for alternative collagen source. It may also be subsequently increase the commercial value of fish.

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Authors: Elham Alsadat Hosseini Aghozbeni, ImaniFooladi Abbas Ali, Mohammad Reza Nourani

In this study, we are focused on developing cell–scaffold constructs to improve bone growth and bone healing. Rat MSCs were isolated from the femurs of rats. The bone marrow suspensions were cultured in the medium consisted of Dulbecco modified Eagle medium supplemented with fetal bovine serum and penicillin–streptomycin. Bioglassnano-composite (BG) scaffold was made through Sol-Gel methodand was evaluated by in vitro cytotoxicity.Osteogenic materials added tothe culture medium and occurrence of differentiation examined by alizarin red staining. The results revealed that BG scaffolds has no toxicity and is biocompatible, following alizarin red staining thered color demonstrated the mineralizing areas of cultures. The present study demonstrates that rMSCs enable to differentiate to bonecell on the Nano bioglass scaffolds.

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Authors: Fatemeh Basiri Basiri, Abbass Ali Imani Fooladi, Masoumeh Foroutan Koudehi, Babak Farzam, Shahnaz Shekarforoush, Mohammad Reza Nourani

Early inflammatory procedures may inhibit functional recovery after nerve injury. Tumor necrosis factor-α (TNF) plays a crucial role in the initiation of degenerative cascades after peripheral nerve injury.The aim of this study was to test the effects of TNF antagonist etanerceptonnerve regeneration in a rat model of sciatic nerve crush injury. 15 adult Wistar rats underwent a surgical procedure involving right sciatic nerve crush injury. Etanercept(6 mg/kg) was administered intra-peritoneally (IP)once immediately after nerve crush in the experimental group. Rats in the control group received saline IP post surgery. Genomic and immune-histochemical tests were done at 1, 3, 7, 21 and 42 days after injury. Functional and electromyography (EMG) assessments of nerves were performed at 7, 21 and 42 days after injury. Functional recovery was analyzed using hot plate test, a walking track assessment and quantified using the sciatic functional index (SFI). TNF mRNA expression was induced at 1 day and returned to baseline at the end of 1 week after injury in nerve. Etanerceptprevented increase of TNF in the crush site and enhanced the rate of axonal regeneration, as determined by increased number of characteristic clusters of regenerating nerve fibers distal to nerve crush segments, but it was not significant statistically with control group on 42 days. The statistical analyses of EMG studies showed that the latency and the amplitude in experimental group of 21 and 42 days were significantly different from the control group. SFI in the experimental group on 21 and 42 days was significantly higher than that of the control group. This study established that immediate therapy with etanercept promotes the regeneration of peripheral nerve injuries in a rat model.