Rybohospodars'ka nauka Ukrayiny (Fisheries Science of Ukraine)

16-27

Authors: M. Sarieva, M. Aliiaskarov, B. Dzhenbaev, A. Sergek uulu

Purpose. The development of a Fish Yield Prediction Model for lakes and water reservoirs of the Kyrgyz Republic based on easily measured physico-chemical and biological indicators (limnological parameters) for the effective management and sustainable use of fish resources. Methodology. Water samples for the analysis were taken once at each season (winter, spring, summer, autumn) during 2014 - 2015. The sampling included the collection of the following parameters: water temperature, acid-base balance of water (pH), electrical conductivity, dissolved oxygen, total dissolved solids (TDS), total alkalinity, nitrates, chlorophyll a. The samples were taken in surface layer of water at a depth of 0.5 m into 250 ml sterile chemical bottles. Chlorophyll "a" concentration was measured by a spectrophotometric method in laboratory conditions, according to a method described by Stirling H.P. The total alkalinity was determined by titration of sodium tetraborate solution (reverse titration), nitrates were determined by a photometric method with the Griss reagent after reduction in the cadmium reducing agent. The measurement of rapidly changing parameters, such as dissolved oxygen concentration (О2) was carried out by portable field equipment “OAKTON” DO 110, acid-base balance (pH) – “OAKTON” PH 11, measurement of water temperature, electrical conductivity, total dissolved solids (TDS) was carried out with the device "Orion Star"A 322. All measurements were taken directly in water areas. Findings. According to data analysis, a number of indices, such as the morpho-edaphic index (MEI), primary productivity and biomass of phytoplankton, are strong indicators of fish yield, both in temperate and tropical lakes and reservoirs. It was identified that with an increase in the morpho-edaphic index, fish yield per unit area increases. Based on the results of our analysis, a Fish yield prediction model in six water bodies of the Kyrgyz Republic was developed, which has the following form: Y = 0.1154 х MEIc 1.1628; Y = 0.5613 х MEIa 1.1786; where: Y- fish yield, kg/ha/year; MEIc - Morpho-edaphic Index of Conductivity; MEIa - Morpho-edaphic Index of Alkalinity. Originality. This is the first research on the empirical Model development of fish yield prediction for lakes and water reservoirs in Central Asian region. The proposed Fish Yield Prediction Model, developed on the basis of MEI, using the statistical method of analysis, has invaluable practical utility to understand the fisheries potential of lakes and water reservoirs in the region, where such environmental conditions prevail. Practical value: If reliable data on fish stock assessment in water bodies are unavailable, the proposed model will be used as one of the tools in the management of fish stocks.

39-49

Authors: M. Pashko, A. Tretiak, S. Pashko, Y. Kolos, N. Mykhaylenko

Purpose. To determine main the technological parameters of artificial reproduction of sterlet (Аcipenser ruthenus) and Siberian sturgeon (Acipenser baerii) with a combined use of industrial aquaculture methods. Methodology. The material for the study were brood sterlet and Siberian sturgeon reared in cage conditions in a water body with natural temperature regime of the forest-steppe of Ukraine. The experiments were performed at a regulated water temperature regime at different periods of the spring (March-May) with the use of natural gonad-stimulating hormones for the stimulation of the maturation of sexual products in fish. During the process of the preparation of brood fish for fish breeding works, a recirculating water supply facility was used. The determination of technological parameters and study of physical-chemical factors of the environment were performed according to generally accepted methods. Findings . At a water temperature of 14-16оС, high efficiency of gonad maturation was observed in sterlet and Siberian sturgeon females with mean weight of 2.8 and 11.6 kg, respectively. The mean weight of eggs obtained from the brood fish was 0.45 kg in sterlet and 1.48 kg in Siberian sturgeon. The mean working fecundity was 43.29 thousand eggs in sterlet and 83.75 thousand eggs in Siberian sturgeon at a relative working fecundity of 15.44 and 7.49 thousand eggs/kg of fish body weight, respectively. During the process of egg incubation, development of fish embryos at the gastrulation stage was on average 87.17% in sterlet and 84.50% in Siberian sturgeon. The output of free embryos of both species from the quantity of embryos on the stage 15 of embryonal development was close to 83-84%. Originality . Peculiarities of the production of embryos from brood sterlet and Siberian sturgeon with a combined use of different methods of industrial aquaculture in the conditions of a sturgeon farm of the forest-steppe physical-geographical zone of Ukraine have been studied. Practical value . The results of the study are of interest for the devolvement of modern methods of sturgeon aquaculture in the conditions of an industrial type farm.

89-102

Authors: R. Palamarchuk, O. Deren

Purpose. Determination of the effectiveness of amaranth assimilation in the body of 1+ carp, the study of the effect of this non-traditional feed component on some physiological and biochemical indices of experimental fish and the economic component of their rearing. Methodology. Fish-rearing and hydrochemical studies were carried out according to generally accepted aquaculture methods. The hemoglobin content in carp blood was determined by the hemoglobin-cyanide method. The number of erythrocytes in carp blood was counted in a Goryaev’s chamber. Protein content determination was carried out according to the Bradford’s method. The concentration of diene conjugates was studied by a method based on the reaction of the optical density of the heptane-isopropanol extract of lipids. The concentration of TBA-active products was determined spectrophotometrically by a color reaction with thiobarbituric acid. The activity of superoxide dismutase (SOD) was determined by the percentage inhibition of the reduction of nitrous tetrazolium in the presence of phenazin methosulfate. The catalase activity was determined based on a change in H2O2 concentration. Findings. The study was carried out under the conditions of the State Enterprise Lviv Experimental Station of the Institute of Fisheries of the National Academy of Sciences. The subject of the study were 1+ Lubin scaly carp. Carp of the first experimental group received amaranth seed flour added to the main diet as 10% at the end of the culture season for 30 days, while another experimental group received 20%. The control group of carp was fed with grain mixtures during the entire growing period. The conditions of fish keeping were identical according to the experiment goal. The temperature regime and main hydrochemical parameters in the experimental ponds were within the limits of the normative values throughout the entire growing season. When feeding 1+ carp with amaranth, a positive effect was obtained for hematological parameters, nutritional properties of meat, activity of antioxidant protection system, and reduction in lipid peroxidation products, namely: an increase in the number of erythrocytes and hemoglobin content (p˂0.05-0.001), a tendency to an increase in protein and fat contents in muscles, increased activity of superoxide dismutase, a decrease in the number of TBA products and diene conjugates in blood serum. Originality. Peculiarities of growth, development of carp and efficiency of using feeds of various compositions with the addition of amaranth seeds against the background of similar satisfactory growing conditions were studied for the first time. At the same time, the effect of amaranth on physiological and biochemical parameters of the body of experimental fish was investigated. Practical value. Based on the analysis of changes in the physiological and biochemical functions of fish organism and economic efficiency as a result of feeding fish with amaranth, quantitative norms and methods for adding it to the diet of carp were developed.

112-117

Authors: Ir. Hrytsynyak, T. Shvets

Purpose. To create a thematic bibliographic list of publications on studies of ecological and biological characteristics of the Black Sea roach (vyrezub) Rutilus frisii frisii Nordman, as an endangered cyprinid species of the fish fauna of Ukraine. Methods. The complete and selective methods were applied in the process of the systematic search. The bibliographic core has been formed with the publications exclusively in Ukrainian and Russian from the fund of the scientific library of the Institute of Fisheries NAAS of Ukraine. Results. There was composed a thematic list of publications with a total quantity of 37 sources covering a time interval from 1931 to 2017 devoted to the questions on the study of the geographical distribution, morphological and ecological characteristics, biological features of different developmental stages, as well as the specifics of reproduction and technologies of pond and industrial rearing of a Red Book fish species, the Black Sea roach, in the context of biodiversity conservation. In addition, the list includes publications on problems of the restitution of this species in river basins. The literary sources are arranged in alphabetical order by author or title, and described according to DSTU 8302:2015 “Information and documentation. Bibliographic reference. General principles and rules of composition”, with the amendments (code UKND 01.140.40), as well as in accordance with the requirements of APA style — international standard of references. Practical value. The list may be useful for scientists, practitioners, students, whose area of ​​interests covers the questions of biological studies, and problems of the restoration and conservation of the Black Sea roach as an endangered object of fish fauna.