Buletin Kebun Raya

75-84

Authors: Florentina Indah Windadri, Dewi Susan

Exploration and collection of mosses have been conducted in Raja Ampat islands (Waigeo, Batanta, and Salawati islands), The result recorded 85 collection numbers of mosses, consist of 56 species that belong to 25 genera and 11 families. Four species namely Calymperes palisotii, Ectropothecium monumentorum, Macromitrium orthostichum, Thuidium tamariscellum have status as new records to New Guinea and Taxithelium bakeri and Taxithelium oblongifolium are two new records to Indonesia .

93-100

Authors: Dyah Subositi, Yuli Widiyastuti

Ekinase (Echinacea purpurea (L.) Moench) a medicinal plant that has immunostimulatory activity. This plant has been cultivating in Tawangmangu region by Medicinal Plant and Traditional Medicine Research and Development Office since 2002. Ten accessions of E. purpurea were found based on their morphological variation, three accessions of them are selected as promising accessions namely BH2, BHU3 dan BHU5. The objective of this research was to observe the genetic diversity of those promising accessions and 8 variants accession from mass selection year I using RAPD analysis. Those accessions were amplified using 10 RAPD primers. A total of 64 scorable fragments were generated from 9 RAPD primers, among which 48 fragments (75%) were polymorphic. The Dice coefficient was used to calculated the genetic similarity and UPGMA was used to generate the dendogram. The genetic similarity index among accessions evaluated ranged from 75,49-84,21% thus indicating that low level of genetic diversity. RAPD analysis proved to be efficient for genetic diversity of ekinase accessions from mass selection year I.

115-130

Authors: Yusi Rosalina, Kuswata Kartawinata, Nisyawati Nisyawati, Erwin Nurdin, Jatna Supriatna

This study was conducted to collect information regarding carbon storage, particularly the carbon stored in the aboveground biomass as well as in the peat soil. The study site was in a conservation area of the PT National Sago Prima belonging to the PT Sampoerna Agro Tbk. Group. The work was undertaken in January-February 2012. The total aboveground biomass and carbon storage in the research site indicates that the conservation area is a secondary peat swamp forest, with the biomass of 149.18 ton/ha and carbon stock of 70.12 ton C/ha. The aboveground biomass and carbon consisted of biomass and carbon of tree (83.97 ton/ha containing 39.47 ton C/ha, respectively), saplings (20.69 ton/ha containing 9.72 ton C/ha) and seedlings and undergrowth plants (0.03 ton/ha containing 0.01 ton C/ha), wood necromass (24.06 ton/ha containing 11.31 ton C/ha), and litter (20.44 ton/ha containing 9.61 ton C/ha). An allometric equation was developed for Pandanus atrocarpus Griff. providing an estimated total biomass and carbon of 5.16 ton/ha containing 2.42 ton C/ha. The underground C with the mean peat depth of 5.5 m, mean bulk density of 0.18 g/cm3, and the mean organic C of 46.6 % was 464.895,94 ton C/ha. In the entire conservation forest area of 541 ha the total aboveground biomassa and carbon storage was estimated to be 80,708.64 ton and 37,934.00 ton C, while the total underground carbon was 238,96 million MT C.

1-16

Authors: Aldi Rahman Hakim, Dorly Dorly, Sri Rahayu

Hoya spp. (Apocynaceae: Asclepiadoideae) has differences in leaf type, there are succulent and non succulent leaf types. Anatomical structure of Hoya spp. leaf has not been widely studied, especially for the non succulent type. The aims of this research were to explore the diversity and relationship of non succulent Hoya, based on leaf anatomical characters represented by eight species i.e. H. bandaensis, H. campanulata, H. chlorantha, H. cilliata, H. coriacea, H. coronaria, H. densifolia and H. multiflora. Hoya leaf anatomical characters were observed on the paradermal and transversal section and analyzed by using IBM SPSS version 19 sotfware for cluster analysis. According to the paradermal observation, stomata were present at lower surface (hypostomatic) for all species, and amphistomatic (both surface) for H. densifolia. The type of stomata is cyclocytic for all species. Clustered stomata were found in H. coriacea. Observation on transversal section showed that all of species has the normal structure i.e cuticula, upper and lower epidermis, palisade parenchyma and spongy parenchyma with the variation in the layer thickness. The cluster analysis resulted four groups at distance scale 19. Each group has specific characters. The first group has trichomes on both sides of the leaf surface. The second group has amphistomatic stomatal. The third group has thin leaves. The fourth group has the widest stomata.

27-36

Authors: Yayan Wahyu C. Kusuma, D.O. Pribadi, Y. Isnaini

Ramin (Gonystylus bancanus) is one of the major timber species that facing high exploitation in Indonesia. This species can only be found on a specific peat swamp habitat, thus it confines its distribution. Information on its current population and microhabitat characteristics is relatively limited. Here, we investigated natural population and microhabitat of ramin in a peat swamp area in Riau Province using random transects consist of 46 (100m2) sampling plots. Forty-eight individuals of ramin were found in which 46 of these belonged to G. bancanus, while the other two were different species. The estimated population density of ramin in this area was 7.18±2.75 individuals/ha i.e there were approximately seven individuals in each one-ha area of study. The population structure of ramin showed a J-shaped curve bearing many large old trees with few saplings without any seedlings. The microhabitat was investigated using the Discriminant Function Analysis (DFA) and Canonical Analysis, generating two significant variables that discriminated three groups of the population.

58-73

Authors: Putra Hafiz, Dorly Dorly, Sri Rahayu

Hoya has two types of leaves, succulent and non succulent. Succulent plant adapted well in extreme conditions, especially during dry period. Anatomical characters can be used to identify, classify, and determine plant relationship. The aims of this research were to identify anatomical character of succulent Hoya’s leaf and to determine their relationship. Ten examined species Hoya spp. (H. diversifolia, H. latifolia, H. dolichosparte, H. bilobata, H. lacunosa, H. verticillata, H. purpureofusca, H. kuhlii, H. oblanceolata, and H. micrantha) showed anatomical similarity in their epidermal layer, hipodermal, mesophyl, and the absence of sponge tissue at mesophyl. However, there are diversities among the species. Based on paradermal section of the leaf, two types of stomata were only found at the abaxial side, individual stomata and both individual and stomatal cluster. Hoya diversifolia has the smallest stomatal size, while Hoya latifolia has the biggest. Hoya lacunosa has the lowest stomatal density, while Hoya bilobata has the highest. Hoya diversifolia, Hoya lacunosa, and Hoya oblanceolata have the lowest stomatal index, while Hoya bilobata has the highest. Observation on transversal section showed that Hoya purpureofusca has the lowest leaf thickness, while Hoya kuhlii has the highest. The hierarchical cluster analysis based on anatomical leaf characters showed different patterns of relationship which diversed as three groups at relationship scale 15.