Studia Universitatis Moldaviae. Stiinte reale si ale naturii

3-9

Authors: Valentina BULIMAGA, Liliana ZOSIM, Maria PISOVA, Valeriu RUDIC* , Sergiu ŞOVA

Spirulina productivity and iron content in biomass recorded higher values at light intensity 5500 lx, compared to 3500 lx, but phycobiliproteins accumulation in spirulina biomass was more significant at 3500 lx. The content of the accumulated selenium in the phycobiliproteins registered the increased values with the increasing of selenite concentra¬tion, attesting its maximum value at 45 mg/l, at the both light intensity. Selenium content in the phycobiliproteins at 3500 lx was by 1.4-1.5 times higher, compared to that registered at 5500 lx.

16-20

Authors: Angela RUDAKOVA., Valentina BULIMAGA, Maria PISOVA, Veaceslav REVA, Natalia CLIMOVA, Sergiu RUDAKOV, Liliana ZOSIM

A comparative evaluation of papain and trypsin hydrolysates of phycocyanin and Se-phycocyanin after separation by HPLC with reverse phase on the ResourceTMRPC column was performed.The chromatographic profile of peptides from the trypsin and papain hydrolysates of phycocyanin at separation by gel filtration on Sephadex G-50 column was analyzed. UV-VIS spectra of peptides fractions obtained by gel filtration separation of phycocyanin hydrolysates revealed the presence of chromopeptides.

25-28

Authors: Galina DOBROJAN

This article reflects a systematization of the results presented in the literature in the field. The research approaches genus Anabaenopsis by studying history, systematic changes, highlighting the number of species and taxa of algae that are assigned of genre and some specific morphological characteristics.

34-38

Authors: Maria DUCA, Angela PORT, Steliana CLAPCO, Adriana ACCIU

The researches both from the scientific publications and three projects included in public databases: Angiosperm Phylogeny Website, version 13, Parasitic Plant Connection, Parasitic Plant Genome Project have been analysed and some correlation between degree of parasitism and genome evolution of Orobancheae have been highlighted. It has been concluded that number of chromosomes, presence of introns, deletion of some regions of plastid genome, provides useful information for studying of genome evolution mechanisms, specific taxonomic affiliations etc.

44-51

Authors: Maria MAGHER

Fire blight, a bacteriosis of fruit-trees and other rosaceous plants, can be a disastrous disease in orchards and is therefore of great economic importance. Many symptoms of fire blight can be seen during short time after infection. Using special microbiological media this pathogenic bacterium has been isolated from samples of infected apple, quince and apple trees. Inoculation tests confirmed its pathogenicity and virulence to host plants.

58-64

Authors: Алла ДОНОС, Светлана ГАРАЕВА, Анна ЛЕОРДА, Влада ФУРДУЙ, Галина ПОСТОЛАТИ, Галина РЕДКОЗУБОВА

The paper highlights the community-acquired children’s pneumonia and recurrent respiratory infections mechanisms of influence in the exchange of free amino acids serum, and shows the qualitative and quantitative amino acid modifications influence in the morphology and function of tissues and organs.

70-75

Authors: Constanţa MADAN

The research purpose is to study and reveal the structure of sexual offenses against minors in Moldova with obvious psychiatric consequences. 100 cases of sexual offenses against children examined at Clincal Department of Forensic Medical Center and at the Archive of the Psychiatric Hospital from Moldova were studied, during the period of 2010-2015. It was established the impact of the sexual abuse to minors according by some points, using an annex statistics.

83-90

Authors: Dumitru BULAT, Denis BULAT, Ion TODERAŞ, Nina FULGA

Under the intensification of anthropogenic pressure in aquatic ecosystems of Republic of Moldova, most of stenobionte species became threatened with extinction, but some species, potentially high adaptive, contrast, in present demonstrates an evident biological progression, in some hydrobiotops causing a stressed invasive effect. For Dniester River (limits of Republic of Moldova) three-spined stickleback – Gasterosteus aculeatus Linnaeus, 1758 is a good example, after the construction of Novodnestrovsk hydropower (1981) became a multidominate species forming to Soroca city a particularly large local population. In this paper are analyzed bio- ecological peculiarities of three-spined stickleback and are highlighted environmental factors that contributed to taxon invasion in Dniester River (limits of Republic of Moldova).

108-113

Authors: Ho Anh TUAN

Analysis and identification of 42 specimens of family Terapontidae collected from Gianh estuary and Gianh downstream in Quang Binh province in north center region Vietnam. We have classified four species: Terapon jarbua (Forsskål, 1775); Helotes sexlineatus (Quoy & Gaimard, 1825); Rhyncopelates oxyrhynchus (Temminck and Schlegel, 1842); Pelates quadrilineatus (Bloch, 1790). Species Rhyncopelates oxyrhynchus (Temminck and Schlegel, 1842) was first discovered in Gianh estuary and Gianh downstream in Quang Binh province in north center region Vietnam.

121-125

Authors: Viorica GLADCHI, Nelli GOREACEVA, Elena BUNDUCHI

In this paper it is presented the results of the multiannual study of the temporal and spatial variations of the organic substances (O.S.) in the Dniester waters on the portion Naslavcea village - accumulation tank from Dubasari. After peculiarities of the regime formation of the Middle Dniester, are highlighted two portions of the river, which differ in content and type of S.O., as well in the degree of pollution of the Dniester waters.

133-141

Authors: Angela LIS

It was studied direct photolysis in aqueous solutions of the thiourea and cysteine on the model system in order to estimate their role in the processes arising in the natural waters. It has been found that both of them, thiourea and cysteine, have been subjected to direct photolysis at irradiation with different artificial light sources. From the results obtained it was found that oxidation rate of these thiols depends directly proportional to the initial concentration of thiol in the system and the emission spectrum of the lamp. There were determined quantum yields of thiourea and cysteine using chemical actinometer K3[Fe(C2O4)3] · 3H2O. It was found that the quantum yield of thiourea is much greater than unity, which shows that on the destruction of thiourea only at the initial stage occurs photochemical reaction, which is followed by chemical reactions, possibly radical reactions, so thiourea can be the initiator of chemical self-purification reactions in the natural waters.

153-157

Authors: Gheorghi NICULIŢA

The presented data confirm the fact that the human society has become one of the factors forming the environment quality. Urban media differ in the ways they influence upon adjacent natural ecosystems. All of them are oriented so as to achieve an ecologic equilibrium that would combine decent living conditions for people from the one hand and a “healthy” environment from the other hand.

164-169

Authors: Ion TODERAŞ, Aurelian GULEA, Elena ROŞCOV, Olga GARBUZ

This work will focus on the study of groups of metalloorganic substances on aquatic organisms. Toxicological testing method of coordination compounds CMT-28 and CMA-18 is used on Paramecium caudatum Ehrnbg ciliates, at concentrations of 100, 10, 1, 0,1, 0,01 uM/L, compared to the prototype. Bioassay method allows us to develop fast and comparative conclusions, avoiding a high risk of errors. The investigation of metalloorganic compounds was performed by the method of serial dilutions in the liquid nutrient medium (1 g nutrient solution / 1l water). As a reference cultures were used ciliates Paramecium caudatum. Infusorians, as objects of research are very comfortable, they reproduce quickly enough, being the most convenient test-objects for study in the laboratory conditions, because of time factor which plays a decisive role and also is more simple to elucidate the impact of abiotic factors (metalloorganic substances) on the process of asexual reproduction of Paramecium caudatum. The activity of substances was evaluated according to the character of the toxicity of the preparations (LT50 and LC50), while the activity of ciliates was studied according to the character of viability and reproductive parameters (increasing or decreasing of numbers and their reproductive rate). The acute toxicity study (LC50 and LT50) of the compounds was performed according to the methodical recommendations on overall toxicity of pharmaceutical remedies. LC50 (lethal concentration, 50%) – concentration which causes death of 50% of test-organisms in a given period of time. LT50 (lethal time, 50%) – time, during which the used concentration of the test compound causes the death of 50% of the test organisms.

178-184

Authors: Tatiana PALAMARCIUC, Angela SÎRBU, Oleg PALAMARCIUC

The synthesis, crystallographic and magnetic studies of Fe(III) compounds with 4-phenylthiosemicarbazone and 4-phe¬nylthiosemicarbazone 2-acetylpyridine are presented. The spectrophotometric studies in solution were realized for Fe(II) ions with the same ligands. The coordination compounds [Fe(L1)2]BF4•CH3OH (1) and [Fe(L2)2]FeCl4 (2) were obtained from alcoholic solutions. Crystallographic studies reveal that the methylation of sulfur atom influence the coordination set on the Fe(III) ion. Compounds 1 and 2 shows different magnetic properties in function of temperature.

189-194

Authors: Roman RUSNAC, Anna RUSNAC, Olga GARBUZ, Nicanor BARBĂ, Aurelian GULEA

In this paper are described methods of synthesis of six organic compounds, were three of then are unknown. The structure of the compounds was determined by NMR spectroscopy 1H, 13C and IR spectra. Anticancer activity was studied using cervical cancer cells HeLa, where the compound N-[4-({2-[1-(pyridine-2-yl)ethylidene]hidrazincarbotioil}amino)phenyl] acetamide showed the highest inhibition, about 84% at concentration of 100 μM/L.

204-213

Authors: Vasile RUSU

It was developed the criterion for delimiting of characteristics for electric double layer (EDL) close to the basal surface and edge surface of pillared Al-montmorillonite, and the EDL characteristics in the Stern model were determined. Depending on the case, for EDL close to the basal surface or edge surface, the main characteristics of Stern layer can be estimated, i.e. the Stern potential , chemical specific adsorption energy Ø and charge density c.

3-9

Authors: Aurelia CRIVOI, Iurie BACALOV, Elena CHIRIŢA, Ana ILIEŞ, Valentin AŞEVSCHI, Lidia COJOCARI, Iulian PARA, Irina BACALOV

In terms of contemporary civilization, man lives in a spacious sound, continually accompanied by a "cortege" noise and vibration of the most varied intensities and effects having more or less aggressive on the comfort and even on his health. Due to its harmful and its presence in all departments of life, noise pollution is a major problem in all countries where we are witnessing a phenomenon of increased noise levels.

15-20

Authors: Ina DIDILICĂ

In this paper there is analyzed the influence of zinc and selenium on male reproduction, as well as the factors that bring to their infertility. In order to overcome the infertility problems, more studies showed that namely the influence of the process of spermatogenesis via the essential factors, participants in spermo-production could regulate the biological properties' growth of sperm. The sperm formation involves a series of molecular and morphological changes in the male germ cells which are represented by the diploid stem cells, the spermatogonia, whose stock is maintained through mitosis, especially in the initial phases of spermatogenesis. Thus, the nutrition supplementation with the above-mentioned mineral elements improve the function and the reproductive system, as well as other vital systems of the organism.

28-31

Authors: Iulian PARA

Diabetes is a disease metabolic chronic what is due to the primary deficit absolute or relative insulin and is characterized by disturbing the predominant carbohydrate metabolism, reduced tolerance to carbohydrates, the presence of glucose in urine, along with impairment and other metabolisms: Lipid and protein.

43-48

Authors: Нина ФУЛГА, Ион ТОДЕРАШ, Дмитрий БУЛАТ, Денис БУЛАТ, Надежда РАЙЛЯН

In the context of middle Dniester the three-spined stickleback female spawn three portions of eggs. The ovulation of the fourth generation of eggs in the current breeding season does not occur due to the lack of conditions for the completion of trophic substances accumulation. Before sweeping the next generation oocytes the value of gonadosomatic index is significantly decreasing (P≥0.95), which indicates a decrease in the relative weight of the ovaries and in the number of yolk oocytes, forming the second and third generation of the eggs of the given breeding season. The value of absolute fecundity of females significantly increases with the length and weight of the fish's body (P≥0.95), while the value of the relative fertility significantly decreases with increasing of age of producers (P≥0.99).

56-60

Authors: Ala CHERDIVARĂ, Angela RUDAKOV, Andrei ŞUTOV

Among seed storage proteins, the peanut 7S globulin Ara h1 exhibits a highest allergenicity. Multiple antigen determinants (IgE epitopes) have been identified inside the Ara h1 amino acid sequence. The presence of potential IgE epitopes inside structurally equivalent homologous sequence regions of 7S globulins from other plants (mainly from soybeans) was shown. Probable existence of a cross-immunoreactivity between Ara h1 and the subunits of soybean 11S globulin Gly m G5 was demonstrated.

72-75

Authors: Alina BEŞLIU, Elena CHIRIŢA, Agafia USATÎI

The present work provides the results of the research of the influence of the inorganic nanoparticles TiO2 and Fe3O4 with the size of 30 nm, in different concentrations, on the growth process and biochemical composition of biomass stalk of the yeast Rhodotorula gracilis CNMN-Y-30. It has been found that nanoparticles at concentrations of 0.5 to 5.0 mg/L did not significantly alter the reproduction of the cells and the productivity of the yeast. The positive effect is manifested by favorable changes in carbohydrate and protein content, at the cultivation in the presence of the nanoparticles TiO2 and Fe3O4.

80-85

Authors: Elena SAŞCO

The following characters have been studied: the length of embryonic root and stem at early ontogenetic stage, the callus frequency of mature embryos, callus area and biomass of autumn common wheat parent genotypes Niconia, Odeschi 267 and reciprocal hybrids F1, F2, BC derived from reciprocal crossover of the genitors. It has been determined that maternal genitor Niconia was involved in the development of growth characters in optimum conditions (witness). On background with culture filtrate H. avenae the resistant genotype Odeschi 267 manifested as a maternal and also as paternal donor for the root and only maternal – for the stem. The hybrids of reciprocal combinations have demonstrated high specificity in phenotypic manifestation of callus frequency and callus area characters, between indexes being established negative correlations in witness and background choices with FC H. avenae. Genotype Niconia has been attested as an efficient genitor for the heredity of callus frequency, while for the callus area character – parent Odeschi 267. In phenotypic expression of callus genesis maternal effect manifested for characters at hybrids F1, F2, while the paternal form influenced the alternative proportion of additive factors in character expression at retro crossover hybrids. Through cluster analysis (dendrograms, k-means) of the growth index and callus genesis, it has ascertain similarities and repartition differences of reciprocal hybrids, the manifestation of genes that control characters – being influenced by FC H. avenae.

91-94

Authors: Irina STRATULAT, Victor ŞALARU, Sergiu DOBROJAN

In this study is presented the biochemical components of algal biomass Nostoc flagelliforme (Berk et Curt) Elenk. cultivated in laboratory conditions in Z-8 medium. The algal biomass has the following chemical content: Mn – 683,9 mg/kg, Zn – 131,5 mg/kg, Pb – <10 mg/kg, Fe – 1,09 g/kg, Na+ - 2,10 g/kg, K+ – 8,01 g/kg, Mg2+ – 5,40 g/kg, Ca2+ – 7,94 g/kg. The highest amount of amino acids are specific for: glutamic acid – 3,8012 mg/100 mg, aspartic acid – 2,7838 mg/100 mg, leucine – 2,4651 mg/100 mg, alanine – 2,3513 mg/100 mg. According to these results the blue-green alga Nostoc flagelliforme can be considered an important potential source of nutritional supplements.

103-107

Authors: Ludmila CUHARSCAIA

According to the Morld Conservation Union, invasive alien species are the second most significant threat to bio¬diversity, after habitat loss. In this article have been generalized and analyzed last data about specific composition of invasive flora from the Chisinau ecosystem, have been studied and analyzed some eco-biological characters of alien invazive plant species. Was assessed their negative and pozitiv impacts on the urban ecosystem, economy and human health.

122-127

Authors: Tatiana DUDNICENCO

In the results of these studies have been elaborated the ecotourism itinerary of „Rudi-Arionesti” landscape reserve, which are representative and accessible for ecotourists: „Rudi” monastery, „La trei cruci” Roman fortress, „Farfuria tur¬cului” medieval settlement, „Raposatilor” cave, „La santuri” Thracian origin fortress, „Valea Bradului”, „Izvorul Verde”. It have been described the main points for visitation within the selected route. It have been emphasized the ecological problems of „Rudi-Arionesti” landscape reservation which represents one of the basic destinations of ecotourism routes from Donduseni and Soroca districts and have been proposed solutions of its solving.

131-143

Authors: Anastasia PAHOLNIŢCAIA

The paper presents a review of the Schiff bases that contain hydrazinobenzothiazole, 4-aminotriazole and lysine and coordination compounds with these ligands described in the literature. 3d metal coordination compounds with these ligands have different composition, structure and properties. Both ligands and coordination compounds manifest antitumor, antibacterial, and antituberculous activities. Copper(II) coordination compounds with these ligands manifest better anti¬tumor activity than corresponding Schiff bases.

151-152

Authors: Viorica Gladchi

Când Omul ajunge la etapa realizărilor multiaspectuale, de regulă, în momentul atingerii unui jubileu, se opreşte puţin din alergătură, se uită la sine dintr-o parte, examinează toate reuşitele, biruinţele (în luptă cu insuccesele), îşi apreciază culmile atinse pe parcursul vieţii, face o com¬paraţie între „ce este” şi „ce ar fi fost dacă”, neapărat se luminează la faţă şi mulţumeşte Domnului pentru ziua de ieri, cea de azi, dar, mai ales, pentru cea de mâine. Acest Om poate fi asemuit cu Aurelian Gulea, academician, doctor habilitat, profesor universitar, şef al Departamentului Chimie la Facultatea de Chimie şi Tehnologie Chimică a USM.