Bulletin of Pharmaceutical Research

112-117

Authors: Arpit Patwari, Manan Dabhi, Ishwarsinh Rathod, Urvish Desai, Bhanubhai Suhagia*, Mukesh Sharma

High performance liquid chromatography and absorbance correction methods were developed and applied for the simultaneous determination of ofloxacin and cefixime. Chromatographic separation was achieved on reversed-phase C18 column(25 cm × 4.6 mm id, 5 µm) in the isocratic mode using methanol-water-triethylamine (25:75:1.5, v/v/v), pH adjusted to 3.50 ± 0.05 with orthophosphoric acid as the mobile phase at a flow rate 0.9 ml/min. Quantitation was achieved with UV detection at 254 nm. In the proposed HPLC method, quantification was achieved over the concentration range of 5-30 and 5-30 µg/ml, with mean recoveries of 99.95±1.31 and 100.36±1.24% for ofloxacin and cefixime respectively. In the absorbance correction method, quantification was achieved over the concentration range of 5-25 µg/ml for both drugs, with mean recoveries of 101.23±0.85 and 100.53±1.40% for ofloxacin and cefixime respectively. Determination was performed at 262.5 and 345.5 nm for ofloxacin and cefixime. The proposed methods were successfully applied for the analysis of synthetic mixtures and pharmaceutical formulations of ofloxacin and cefixime without any interference from common excipients. The results obtained by applying the proposed methods were statistically compared by the Student’s t-test.

124-128

Authors: Amaresh Prusty*

The objective of present study was to formulate and evaluate mouth dissolving tablets (MDTs) of poorly water soluble drug amlodipine by incorporating its inclusion complex with hydroxypropyl-beta-cyclodextrin (HP-beta-CD). The formulation of MDTs employed superdisintegrants crosspovidone and Ac-Di-Sol in different concentrations among which F3, consisting of 8 per cent of crospovidone was found to be the best formulation, as it exhibited minimum disintegration time (15 sec) and better drug release profile as compared to other formulations.

133-139

Authors: Joysree Das, Md. Muradur Rahman*

The present study was designed to investigate antioxidant and thrombolytic properties of chloroform extract of Bacopa monniera (L.), along with phytochemical study for the presence of chemical constituents. Antioxidant potential was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging assays. In DPPH scavenging method, scavenging of DPPH was observed in different concentrations (20, 40, 60, 80, 100, 200, 400, 800 μg/ml). Plant extract found to demonstrate significant scavenging activity. Chloroform extract of Bacopa monniera (L.) showed significant clot lytic properties in different blood samples. The mean percent clot lytic activity of chloroform plant extract of Bacopa monniera (L.) was found to be 48.39%.