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ZINC-INDUCED BIOCHEMICAL ALTERATIONS IN THE LIVER OF COMMON CARP, CYPRINUS CARPIO
Authors: Syed Shahid Ali, Muhammad Ayub Qureshi, Muhammad Javed Iqbal and Abdul Rauf Shakoori
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Fresh water fish, Cyprinus carpio was exposed to zinc chloride (50 g/ml) for 48 hours (short term experiment) and 4 weeks (long term experiment). The liver samples were taken out at 6, 12, 24 and 48 hours in first case and at 1,2,3, and 4 weeks duration in second case, and analysed for biochemical changes. In short-term experiment hepatic AP, AcP and GOT activities decreased significantly at 12, 24 and 48 hours zinc chloride treatment. The GPT activity showed decrease (83% and 64%) at 6 and 12 hours while LDH activity was inhibited (56%) only at 6 hours and showed recovery in remaining experimental period. Glycogen content increased (54% and 106%) while glucose showed significant decrease (43% and 45%) at 24 and 48 hours zinc exposure. RNA content increased 32%, 17% and 24% at 12, 24 and 48 hours treatments, respectively. in long-term experiment all the enzyme activities remained unchanged following zinc treatment, except LDH activity which was decreased (70 and 62%) at 1 and 2 week treatments. Prominent decrease (27, 24 and 38% at 1,2 and 3 weeks) in glycogen content and increase (37 and 41% at 1 and 2 weeks, respectively) in glucose content was also observed. Free amino acids showed 56, 34 and 41% increase at 2,3 and 4 week treatments. Hepatic proteins also showed slight alterations.