Ayurvedic formulations are gaining importance nowadays as they are economic, easily available and relatively free from side effects. It is important to bring the use of these remedies in existing frame work of scientific usage. Considering this, ayurvedic formulation “Balachturbhadrika churna” was standardized by using morphological, microscopical, physicochemical, phytochemical and chromatography parameters. Raw materials were checked for standardizing the formulation. Three market samples were collected and compared with prepared formulation.