International Journal of Pharmaceutical Chemistry and Analysis

54-58

Authors: Nita Yadav, Anju Goyal.

Teneligliptin was estimated in tablet form by ultraviolet-spectrophotometric methods followed by distilled water as solvent. Here three methods were used for quantitative estimation of teneligliptin in tablet dosage form. Quantitative estimation of pure drug solution was done at λmax of 244 nm for method I. For measurement of response in method II peak minima of 266.4 nm was selected. Wavelength range of 238.6-247.8 nm was selected for calculating area in area under the curve method. Validation of the methods was performed according to guidelines of International Conference on Harmonisation. The % assay of the formulation by the three methods was in the range of 100.17-100.74. Graph was linear in the range of 5-70 µg/mL for zero order and AUC techniques, while 5-80 µg/mL for first order derivative technique. Good correlation between response and concentration was found as the value of regression coefficient (R2) was 0.999. The accuracy of the drug was ranged in between 98.54-101.80 for all ultraviolet-spectrophotometric methods and was in acceptable range. The percentage RSD values for method precision for all the methods were within the limit of ≤ 2. From the data it was concluded that the methods developed have scope to be applied for quantitative estimation of pure drug of teneligliptin in pharmaceutical dosage form.

66-69

Authors: Arati Prabhu.

Introduction: Molecular dynamics, in which molecules are allowed to interact over time at a given temperature following the laws of classical mechanics has been shown to be highly successful in simulating various biological phenomena down to atomic detail. When applied to complexes of protein targets and potential binders, this technique, provides a detailed description of the stability of the protein-ligand complexes, thereby throwing light on the binding potential of the ligands to probable target proteins. Materials and Method: In the present study we have explored the mechanism of protein synthesis inhibition by the antibiotic thiostrepton with the combination of manual docking followed by molecular dynamics simulations to two molecular targets – the L11/23S-rRNA interface and the elongation factor Ef-Tu. Cross-docking runs on the two native co-crystallised ligands of the target proteins were done as a further probe. Results: Docking of thiostrepton at the L11/23S-rRNA interface as well as Ef-Tu indicated stable binding during 10-ns molecular dynamics simulations, whereas LFF571 binds stably only to its native protein EF-Tu which is in accordance to literature reports. Thus, molecular dynamics simulation studies indicate that thiostrepton has binding potential to two targets of protein synthesis translation, the elongation factor (Ef-Tu) and L11 protein and 23S-rRNA interface. Conclusion: This study corroborates that thiostrepton inhibits Ef-Tu in addition to the L11-ribosomal RNA complex. Binding to multiple targets probably enhances its inhibitory effect on protein translation.

79-82

Authors: Prachi Pimple.

Introduction: Aim of the study is to develop simple, specific, accurate HPTLC method for simultaneous determination of the Dexibuprofen and Tramadol HCl in pharmaceutical dosage form. Materials and Method: Stationary phase was precoated silica gel 60 F254. The Mobile Phase used was mixture of Chloroform: toluene: ethanol: glacial acetic acid (8:4:1:0.5 v/v/v/v). The detection of the spots was carried out at 264nm and 271nm for Dexibuprofen and Tramadol HCl respectively. Result: The method was validated in terms of linearity, accuracy, precision and specificity. The Limit of detection for Dexibuprofen and Tramadol HCl was found to be 1µg and 0. 2 µg respectively. The Limit of quantification for Dexibuprofen and Tramadol HCl was found to be 1.6µg and 0.4µg respectively. Conclusion: The method is free from the interferences due to excipients present in the formulation and can be successfully used for determine drug in pharmaceutical dosage form.