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Optimization and Production of Botryococcus braunii Biomass using Commercial Nutrients by Response Surface Methodology
Authors: C. Kurinjimalar, G.Kavitha, S. S. Begum , G. Rajaram, S.Nagaraj, N. Senthilkumar and R. Rengasamy
Number of views: 350
Biofuel production by a sustainable method using microalgae is entirely dependent on biomass
production. However, commercialization at large scale using microalgae is a major obstacle using
analytical grade growth nutrients, due to their cost effectiveness. Hence, development of a cost
effective method is essential to reduce the production cost. Therefore, the present study envisaged the
effect of low-cost commercial fertilizers such as urea, sodium bicarbonate, magnesium sulfate, potash
and di-ammonium phosphate as growth nutrients for the production of biomass and total lipid of
Botryococcus braunii were made. The biomass and total lipid production were optimized using
Response Surface Methodology by 2
5
Central Composite Design. The result showed 225 mg L
-1
of
urea, 650 mg L
-1
of sodium bicarbonate, 225 mg L
-1
of magnesium sulfate, 150 mg L
-1
of potash and
15 mg L
-1
of di-ammonium phosphate supported the algal growth with a maximum biomass and total
lipid of 0.792 gL
-1
dry wt.. and 260 mg L
-1
dry wt.., respectively. The biomass productivity of alga B.
brauniiat the above condition recorded as 0.04 gL
-1
day
-1
with a generation time of 1.90 days.