International Journal of Integrative Medical Sciences

242-247

Authors: Shaik Hussain Saheb, Desai SD, Kusal K Das, Haseena S.

Introduction: Malondialdehyde (MDA) cause toxic stress in cells and form covalent protein and its used as a biomarker to measure the level of oxidative stress. There is opposite mechanism which control the flux of reactive oxygen species (ROS) called as antioxidant system, which includes both enzymatic andnon-enzymatic components. The important enzyme of this system is superoxide dismutase (SOD). The black cumin or Nigella Sativaseeds are used in traditional medicine of different countries. Thymoquinone is major bioactive component of nigellasativa seed and it is the cause of therapeutic property of Nigella sativa seed. The present study is conducted to see the effect of Nigella Sativa seed powder and Thymoquinone on Serum MDA and SOD levels in normal and Streptozotocine Induced Diabetic Rats. Materials and Methods: 36 rats were selected for this study and divided in to 6 groups each contains 6 rats, one group served as normal control, one group served as normal rats received the nigella sativa seed powder(300mg/Kg BW), one groups served as normal rats received the Thymoquinone(4mg/kg BW), one group served as Streptozotocine(50mg/kg BW) induced diabetic control rats, one group served as diabetic rats received the nigella sativa seed powder(300mg/Kg BW) and one groups served as diabetic rats received the Thymoquinone(4mg/kg BW). Results: There is no change in MDA and SOD levels in normal rats treated with nigella sativa seed powder and thymoqinone. The levels of MDA are increased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the MDA levels are decreased significantly(p<0.05). The levels of SOD are decreased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the SOD levels are increased significantly. Conclusion: The results of these study concluding that nigella sativa seed powder and thymoquinone has having antioxidant effect in diabetic rats which lowering the MDA levels and increasing the SOD levels in streptozotocine induced diabetic rats. KEY WORDS: MDA, SOD, Nigella Sativa seed, Antioxidant. Thymoquinone.

253-256

Authors: Rajoor UG, Mahabalshetti AD, Dhananjaya M.

Background and objectives: Mucormycosis is a relatively rare opportunistic fungal infection. It is one of the devastating infections of immunocompromised host. Though several studies were done in India and elsewhere on Mucormycosis, it has not been extensively studied of late. The objective of the study is to study the clinical profile of Mucormycosis in the northern part of Karnataka. Materials and Methods: Our series compromised of 14 cases seen over a period of 5 years. Detailed history, clinical examination, laboratory investigations were carried out in all the cases. Results: The study group consisted of 10 males and 4 females aged 20 – 70 years (mean 50 years). Most of the patients in the study group had evident blackish nasal eschar and sinus disease. Cultures were positive for Mucorales in 08 cases. 11 patients were treated with amphotericin B in the doses ranging from 0.5 – 1.0 mg/kg up to a total of 2.6 grams, and duration of the treatment varied from 1 – 31 days. Conclusions: Mucormycosis is a rare opportunistic fungal infection with rapidly progressive and fulminant course with often fatal outcome. A strong suspicion, prompt diagnosis with pathological confirmation and aggressive surgical treatment gives a better outcome. KEY WORDS: Mucormycosis, Fungal, Amphotericin.