Effect of Nigella Sativa seed and Thymoquinone on Reproductive Parameters in Sterptozotocine Induced Diabetic and Normal Male Albino Rats
Authors: Manjunath Aithal, Haseena S, Kusal K Das, Shaik Hussain Saheb.
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occur. Antioxidant components in medicinal herbs such as Nigella sativa have been indicated to improve spermatogenesis and steroidogenesis. Thymoquinone and unsaturated fatty acids are the main antioxidant components of Nigella Sativa. Nigella Sativa seeds has been used in traditional Iranian medicine as a natural remedy for promotes females menstruation, galactagogue, carminative, laxative and anti-parasitic properties. The present study is conducted to find out effect of Nigella sativa seed and Thymoquinone on testosterone and LH levels in streptozotocine induced diabetic and normal male albino rats.
Materials and Methods -36 rats were selected for this study and divided in to 6 groups each contains 6 rats, one group served as normal control, one group served as normal rats received the nigella sativa seed powder(300mg/Kg BW), one groups served as normal rats received the Thymoquinone(4mg/kg BW), one group served as Streptozotocine(50mg/kg BW) induced diabetic control rats, one group served as diabetic rats received the nigella sativa seed powder(300mg/Kg BW) and one groups served as diabetic rats received the Thymoquinone(4mg/kg BW).Testosterone and LH were estimated by Chemiluminosis(CLIA).
Results: There is no change in Testosterone and LH levels in normal rats treated with nigella sativa seed powder and thymoqinone. The levels testosterone and LH are decreased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the Testosterone levels are normalised significantly(p<0.05).
Conclusion: Compared with normal rats the level of testosterone was decreased in diabetic rats, when it is treated with Nigella sativa seed and Thymoquinone the levels of testosterone increased significantly. Compared with normal rats the level of LH was decreased significantly in diabetic rats, when it is treated with Nigella sativa seed and `Thymoquinone the levels of LH not significant with other groups.
KEY WORDS: Testosterone, LH, STZ, Diabetes, Thymoquinone, Nigella Sativa seed.