143-172
Studying Physiological, Morphological, and Cytological Alterations in Prokaryotic and Eukaryotic Cells in Heavy Water
Authors: Oleg Mosin, Ignat Ignatov, Dmitry Skladnev, Vitaly Shvets

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In this paper the biological influence of deuterium on cells of various taxonomic groups of prokaryotic and eucaryotic microorganisms realizing methylotrophic, chemoheterotrophic, photo-organotrophic, and photosynthetic ways of assimilation of carbon substrates (methylotrophic bacteria Brevibacterium methylicum, chemoheterotrophic bacteria Bacillus subtilis, photo-organotrophic halobacteria Halobacterium halobium, and green algae Chlorella vulgaris) was investigated at the growth on media with heavy water (2H2O). For all investigated microorganisms are submitted data on growth and adaptation on growth media containing as sources of deuterated substrates 2H2O, [2H]methanol and hydrolysates of deutero-biomass of methylotrophic bacteria B. methylicum, obtained after the multistage adaptation to 2H2O. The qualitative and quantitative composition of intra- and endocellular amino acids, proteins, carbohydrates and fatty acids in conditions of adaptation to 2H2O is investigated. It is shown, that the effects observed at adaptation to 2H2O, possess a complex multifactorial character and connected to physiological, morphological and cytological alterations – the magnitude of the lag-period, time of cellular generation, output of biomass, a parity ratio of synthesized amino acids, proteins, carbohydrates and lipids, and also with an evolutionary level of the organization of the investigated object and the pathways of assimilation of carbon substrates.