Effects of Mangifera Indica Leaf Extracts on the Biochemical Indices of the Liver Function and Some Haematological Parameters in Rabbits
Authors: I.A.A. Ibrahim, M. Chindo
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Research was conducted to study the effect of Magnifera indica dried leaves on two important biochemical indices of the liver, (AST and ALT). Portion of the fractions each was subjected to phytochemical screening for the presence of secondary metabolites including, flavonoids, saponins, tannins and steroids/triterpenes and alkaloids using standard procedures (Silver et al., 1998). A total of 8 rabbits containing four young and four adult rabbits of either sex (2kg) were used for the study. The test animals were divided into four groups of two rabbits each (containing a mixture of one young and one adult rabbit each). T1 serves as the control, T2, T3 and T4 serving as the test groups. The extract was administered orally to the test groups (T2, T3 and T4) orally and distilled water (placebo) was administered orally to the control group (T1). The dosage of administration sustained was 200ml/kg daily in divided doses for a month. The ALT & AST test procedure was conducted using RANDOX reagent according to the manufacturer’s instruction. Results of preliminary phytochemical of the ethanol (EE) leaf extract of M. indica revealed the presence of all the constituent tested including alkaloids, flavonoids, anthraquinones, saponins, steroids, triterpenes, tannins except carbohydrates and glycosides. The aqueous extract (AE) revealed the presence of all the constituents except steroids, triterpenes, anthraquinones, tannins, carbohydrates and glycosides. The results obtained for alanine amino transferases (ALT) and aspartate amino transferases (AST) were found to be within the normal range of 10-45V/L for ALT and 10-120V/L for AST. The packed cell volume (PCV) and hemoglobin concentration were the only heamatological parameters tested. According to the result obtained in the analysis, there was a slight variation between the PCV and Hb concentration in the test animal and the control . Hence, the extract has no adverse effect on the circulating red blood cell as well as the Hb concentration but rather brings about the slight increase in the production of red blood cell as well as the Hb concentration. This may be attributed to the presence of active constituent that promote red cell production in the plant extracts.