Indonesian Journal of Animal and Veterinary Sciences

174 - 181

Authors: Elizabeth Wina, B Tangendjaja, T Pasaribu, T Purwadaria

Utilization of jatropha seed meal as a feed ingredient is limited by the presence of several anti nutritive and toxic compounds in the seed meal. The aim of this research is to evaluate feeding of jatropha seed meal detoxified using fermentation by two fungi and rumen microbes (as biological detoxification) and using a combination of chemical and physical treatments on broiler performance. One hundred seventy five chicks (7 days old) were used and were divided into 5 treatments in 7 replications and each replication in one cage consisted of 5 chicks. R1 was control feed (K) without jatropha seed meal., 2) R2 was feed with 4% of Jatropha seed meal fermented by Neurosphora sitophila (FNS), R3 was feed with 4% of Jatropha seed meal fermented by Aspergillus oryzae (FAS), R4 was feed with 4% of Jatropha seed meal fermented by rumen microbes and R5 with 4% of Jatropha seed meal treated by autoclaved, refluxed by hexane and soaked in methanol (OEHM). Treated feed was given for 14 days at the end of the feeding treatment, two chickens from each replication were slaughtered and organ weights were recorded. Body weight of chicken and feed conversion ratio were calculated. The rest of the chicken was fed commercial feed for the next 7 days (recovery periode). Chicken mortality was almost 0% but 1 chicken from FAS treatment died at the recovery period. Feed consumption was lower at fermented jatropha seed meal than control (K) or OEHM, resulted in lower body weight of chicken. The abdomen fat weight and organ weights especially pancreas or spleen resulted from treatment with jatropha were much lower than that of K. In the recovery period, body weight of chicken in the OEHM treatment was almost similar from that of control chicken. In conclusion, biological detoxification on BBJ was able to reduce chicken mortality but could not improve the daily gain higher than control treatment. The best method to detoxify jatropha seed meal was the combination of physical and chemical treatment (using autoclave, followed by hexane and methanol extractions). Key Words: Broilers, Jatropha Seed Meal, Detoxification, Fermentation

188 - 195

Authors: Andi Tarigan, L Abdullah, S.P Ginting, I.G Permana

One of the main constraints in increasing livestock productivity in the tropical region is the scarcity of good quality feed throught the year, particularly during the long-dry season. Being a leguminous tree, Indigofera sp known to be tolerant to dry climate and salinity, should be considered as alternative fodder for ruminant animals. This study is aimed to investigate the productivity and some nutritional quality of Indigofera sp under different interval and hight of defoliation. The experiment was designed in a randomized block of factorial treatments. The factors were interval of defoliation (30, 60 and 90 days) and hight of defoliation (0.5, 1.0 and 1.5 m above ground level). Indigofera sp was planted in 4 x 3 m plots of 36 plots (4 replications) and were harvested at 8 months old. The DM production, chemical composition and in vitro digestibility were measured according to the treatments. The highest DM productivity was (33.25 t ha-1 y-1) at defoliation interval of 90 days and defoliation hight of 1.5 m, and was not different (P > 0.05) from the treatment combination of 60 days defoliation interval and 1,5 m defoliation hight (31.23 t ha-1 y-1). The highest leaf/stem ratio was 2.6 (P < 0.05) in the 30 day defoliation interval across the defoliation hight. The OM content of Indigofera sp was not affected by defoliation treatments and ranging from 884.6 to 906.8 g/kg. The highest NDF and ADF contents were (P < 0.05) in the 90-d defoliation interval and 1.0 m (387.9 and 272.6 g/kg, respectively) and were not different (P > 0.05) for the 90-d defoliation interval and 1.5 m defoliation hight (385.6 and 267.0 g/kg, respectively). The crude protein content was lowest (P < 0.05) in the 30-d defoliation interval across the defoliation hight ranging from 211.2 to 219.7 g/kg, and it was not different between the 60 and 90-d defoliation interval across the defoliation hight (255.0-258.1 g/kg and 230.3-236.0 g/kg, respectively). Defoliation hight did not affect (P > 0.05) the DM and OM in vitro digestibility. They were at the 90-d defoliation interval ranging from 680.2 to 706.8 g/kg and from 668.6 to 686.8 g/kg, respectively across the defoliation hight different. The DM and OM digestibility were not different (P > 0.05) between the 30 and 60-day defoliation intervals. It is concluded that when planted at low land of wet climate the combination of a 60-d defoliation interval and 1.5 m defoliation hight is an optimum cutting management in utilization of Indigofera sp as foliage for ruminant. Key Words: Defoliation, Nutritive Quality, Productivity, Indigofera sp

205 - 214

Authors: Achmad Fanindi, B.R Prawiradiputra, L Abdullah

Kalopo (Calopogonium mucunoides) was used as cover crops in plantation. Besides, kalopo can be used as forage, esspecially in dry season. The aim of this study was to evaluate the effect of light intensity on forage and seed production. Research was conducted at Research Institute for Animal Production, Ciawi Bogor and Laboratory of Agrostology Faculty of Animal Husbandry, Bogor Agricultural University, for 16 months. Four levels of light intensity, namely: 100%, 80%,60% and 40% were applied, leguminous species of kalopo (Calopogonium mucunoides), was used. Block Randomized Design with 3 replications was applied in this research. Data collected was analyzed by ANOVA and Duncan’s Multiple Range Test. Forage production was evaluated within one year. The forage quality and digestibility (invitro) were investigated. Seed production was accumulated as seasonal seed production during one year. The results showed that light intensity affected (P < 0.05) forage and seed production, chlorophyll and total chlorophyll of kalopo, but did not affect (P > 0.05) quality and digestibility of kalopo. Seed production of kalopo was affected (P < 0.05) by light intensity, the highest forage and seed production of kalopo were obtained from treatment of full light intensity (100%). The seed quality of kalopo was affected by light intensity. The best seed quality of kalopo was achieved from 80% light intensity. The result shows that plots with light intensity of 100% was the best light intensity for forage and seed production of kalopo. Kalopo could grow well in the plots up to 80% light intensity. Key Words: Light Intensity, Forage, Seed, Kalopo

220 - 230

Authors: Bambang Ngadji Utomo, R.R Noor, C Sumantri, I Supriatna, E.D Gunardi

The information of Katingan cattle as an indigenous cattle in Central Kalimantan is very limited, especially phenotypic characterization. A number of 72 heads, consist of 57 heads female and 15 heads male of Katingan cattle in adults category were used in this study. The samples were obtained from three sub districts in district of Katingan, those were Tewah Sanggalang Garing (Pendahara Village), Pulau Malan (Buntut Bali Village) and Katingan Tengah (Tumbang Lahang Village). The location were used as the variable of groups (subpopulation). This study was conducted to describe morphometric performance, which was analysed by discriminant and canonical to estimate the phylogenic relationship and to determine the discriminant variable among the group (subpopulation). The SAS package program version 6.0 and MEGA 2 program was used to analyze data. The observed parameters were body length, wither height, chest width, chest depth, chest circumference, hip heigth, hip width, head height, head length and head width. The chest depth and hip height of female cattle from group of Buntut Bali were lower (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The parameter of: chest width, hip width and body length of Buntut Bali groups were higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The average body weight of male Katingan cattle from Buntut Bali was higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups but there were not different (P > 0.05) on bodyweight of female Katingan cattle among location. The highest similarity value was Katingan cattle from group of Tumbang Lahang (92,86%) followed by group of Pendahara (40.91%) and group of Buntut Bali (38.89%), repectivelly. Katingan cattle from group of Pendahara and Tumbang Lahang had closest genetic distance (1.406), while the longest genetic distance (2.046) was between group of Buntut Bali and group of Tumbang Lahang. Genetic distance between group of Buntut Bali and Pendahara was 1.929. Katingan cattle from Pendahara and Tumbang Lahang within one cluster. Body length in canonical structure 2 can be used as discriminant variable. Key Words: Katingan Cattle, Morphometric, Genetic Distance, Discriminant Analysis, Canonical

240 - 251

Authors: Risza Hartawan, Karl Robinson, Timothy Mahony, Joanne Meers

Hemagglutinin and neuraminidase are the main antigenic determinants of highly pathogenic avian influenza (HPAI) virus. The features of these surface glycoproteins have been intensively studied at the molecular level. The objective of this research was to characterise the genes encoding these glycoproteins by sequencing of multiple clones. The H5 and N1 genes of isolate A/duck/Tangerang/Bbalitvet-ACIAR-TE11/2007 were each amplified in one or two fragments using reverse transcriptase-PCR (RT-PCR), and subsequently cloned into pGEM-T Easy TA cloning system. The sequencing result demonstrated high homology between respective clones but with several variations that were identified as single nucleotide polymorphisms (SNPs). A total of 1,707 base pair and 1,350 base pair of H5 and N1 genes respectively were successfully assembled from multiple clones containing the genes of interest. The features of both H5 and N1 genes from this isolate resemble the typical characteristics of Indonesian strains of H5N1 virus from sub-clade 2.1.3. Key Words: Avian Influenza, Characterization, Gene Cloning, Hemagglutinin, Neuraminidase